Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity

被引：35

作者：

Gaster, Richard S. ^{[2
,3
]}

Hall, Drew A.

Wang, Shan X. ^{[1
,4
]}

机构：

[1] Stanford Univ, Geballe Lab Adv Mat, Dept Elect Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[3] Stanford Univ, Med Scientist Training Program, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA

来源：

NANO LETTERS | 2011年 / 11卷 / 07期

基金：

美国国家科学基金会;

关键词：

Biosensors; nanobiotechnology; antibody cross-reactivity; giant magnetoresistance; magnetic nanoparticles; SPIN-VALVE SENSORS; MICROARRAYS; PROTEOMICS; ANTIGEN;

D O I：

10.1021/nl1026056

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.

引用

页码：2579 / 2583

页数：5