Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity

被引：35
作者：
Gaster, Richard S. [2 ,3 ]
Hall, Drew A.
Wang, Shan X. [1 ,4 ]
机构：
[1] Stanford Univ, Geballe Lab Adv Mat, Dept Elect Engn, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
[3] Stanford Univ, Med Scientist Training Program, Sch Med, Stanford, CA 94305 USA
[4] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
来源：
NANO LETTERS | 2011年 / 11卷 / 07期
基金：
美国国家科学基金会;
关键词：
Biosensors; nanobiotechnology; antibody cross-reactivity; giant magnetoresistance; magnetic nanoparticles; SPIN-VALVE SENSORS; MICROARRAYS; PROTEOMICS; ANTIGEN;
D O I：
10.1021/nl1026056
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.
引用
收藏
页码：2579 / 2583
页数：5
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