Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape

被引：0

作者：

Suryadevara, Naveenchandra ^{[1
]}

Gilchuk, Pavlo ^{[1
]}

Zost, Seth J. ^{[1
]}

Mittal, Nikhil ^{[3
]}

Zhao, Li Leyna ^{[3
]}

Crowe Jr, James E. ^{[1
,2
,4
]}

Carnahan, Robert H. ^{[1
,4
]}

机构：

[1] Vanderbilt Univ, Med Ctr, Vanderbilt Vaccine Ctr, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Med Ctr, Dept Pathol Microbiol & Immunol, Nashville, TN 37232 USA

[3] Agilent Technol, 6779 Mesa Ridge Rd,Suite 100, San Diego, CA 92121 USA

[4] Vanderbilt Univ, Med Ctr, Dept Pediat, Nashville, TN 37232 USA

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 02期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2).For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021).

引用

页数：17

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