Construction of CLEAs-lipase on magnetic graphene oxide nanocomposite: An efficient nanobiocatalyst for biodiesel production

被引：77

作者：

Badoei-dalfard, Arastoo ^{[1
,2
]}

Karami, Zahra ^{[1
]}

Malekabadi, Saied ^{[1
]}

机构：

[1] Shahid Bahonar Univ Kerman, Dept Biol, Fac Sci, Kerman, Iran

[2] Shahid Bahonar Univ Kerman, RTIPP, Kerman, Iran

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 278卷

关键词：

Lipase; Immobilization; CLEAs; Biodiesel; Magnetic; Nanobiocatalyst; CANDIDA-RUGOSA LIPASE; IMMOBILIZED LIPASE; SOYBEAN OIL; BIOCATALYST; PARTICLES; CELLULASE; SHELL;

D O I：

10.1016/j.biortech.2019.01.050

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In the present work, cross linked enzyme aggregates of Lipase (CLEAs-lip) was synthesized and immobilized on the magnetic amino functionalized graphene oxide (maGO-CLEAs-lip) nanocomposites. The immobilized lipase showed a broad range of temperature activity about 40-60 degrees C, as compared to free enzyme. In the case of maGO-CLEAs-lip nanocomposite, the observed lower Km value state 2.25 folds affinity for the p-nitrophenyl palmitate. Enzyme activity of maGO-CLEAs-lip nanocomposite was the highest up to 5 cycles. Storage stability results displayed maGO-CLEAs-lip retained about 75% of its original activity after 30 days of incubation. Remarkably, maGO-CLEAs-lip formed the highest biodiesel construction (78%) from R. communis oil after 24 h of incubation. The biodiesel yield of this nanocomposite was 3.0 folds higher than free enzyme, making it talented as an excellent nanobiocatalyst for efficient production of biodiesel.

引用

页码：473 / 476

页数：4

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