Construction of CLEAs-lipase on magnetic graphene oxide nanocomposite: An efficient nanobiocatalyst for biodiesel production

被引：74
作者：
Badoei-dalfard, Arastoo [1 ,2 ]
Karami, Zahra [1 ]
Malekabadi, Saied [1 ]
机构：
[1] Shahid Bahonar Univ Kerman, Dept Biol, Fac Sci, Kerman, Iran
[2] Shahid Bahonar Univ Kerman, RTIPP, Kerman, Iran
来源：
BIORESOURCE TECHNOLOGY | 2019年 / 278卷
关键词：
Lipase; Immobilization; CLEAs; Biodiesel; Magnetic; Nanobiocatalyst; CANDIDA-RUGOSA LIPASE; IMMOBILIZED LIPASE; SOYBEAN OIL; BIOCATALYST; PARTICLES; CELLULASE; SHELL;
D O I：
10.1016/j.biortech.2019.01.050
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In the present work, cross linked enzyme aggregates of Lipase (CLEAs-lip) was synthesized and immobilized on the magnetic amino functionalized graphene oxide (maGO-CLEAs-lip) nanocomposites. The immobilized lipase showed a broad range of temperature activity about 40-60 degrees C, as compared to free enzyme. In the case of maGO-CLEAs-lip nanocomposite, the observed lower Km value state 2.25 folds affinity for the p-nitrophenyl palmitate. Enzyme activity of maGO-CLEAs-lip nanocomposite was the highest up to 5 cycles. Storage stability results displayed maGO-CLEAs-lip retained about 75% of its original activity after 30 days of incubation. Remarkably, maGO-CLEAs-lip formed the highest biodiesel construction (78%) from R. communis oil after 24 h of incubation. The biodiesel yield of this nanocomposite was 3.0 folds higher than free enzyme, making it talented as an excellent nanobiocatalyst for efficient production of biodiesel.
引用
收藏
页码：473 / 476
页数：4
相关论文
共 24 条
  • [1] Azadian Fatemeh, 2017, Journal of Genetic Engineering and Biotechnology, V15, P187, DOI 10.1016/j.jgeb.2016.12.005
  • [2] Azadian F, 2016, MOL BIOL RES COMMUN, V5, P143
  • [3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [4] Co-immobilization of cellulase and lysozyme on amino-functionalized magnetic nanoparticles: An activity-tunable biocatalyst for extraction of lipids from microalgae
    Chen, Qingtai
    Liu, Dong
    Wu, Chongchong
    Yao, Kaisheng
    Li, Zhiheng
    Shi, Nan
    Wen, Fushan
    Gates, Ian D.
    [J]. BIORESOURCE TECHNOLOGY, 2018, 263 : 317 - 324
  • [5] Enzyme shielding by mesoporous organosilica shell on Fe3O4@silica yolk-shell nanospheres
    Cui, Jiandong
    Sun, Baoting
    Lin, Tao
    Feng, Yuxiao
    Jia, Shiru
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 117 : 673 - 682
  • [6] PREPARATION OF GRAPHITIC OXIDE
    HUMMERS, WS
    OFFEMAN, RE
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (06) : 1339 - 1339
  • [7] Superparamagnetic enzyme-graphene oxide magnetic nanocomposite as an environmentally friendly biocatalyst: Synthesis and biodegradation of dye using response surface methodology
    Kashefi, Saeed
    Borghei, Seyed Mehdi
    Mahmoodi, Niyaz Mohammad
    [J]. MICROCHEMICAL JOURNAL, 2019, 145 : 547 - 558
  • [8] Magnetic cross-linked enzyme aggregates (MCLEAs) applied to biomass conversion
    Lucena, Guilherme Nunes
    dos Santos, Caio Carvalho
    Pinto, Gabriel Cardoso
    da Rocha, Caroline Oliveira
    Brandt, Joao Victor
    de Paula, Ariela Veloso
    Jafelicci Junior, Miguel
    Costa Marques, Rodrigo Fernando
    [J]. JOURNAL OF SOLID STATE CHEMISTRY, 2019, 270 : 58 - 70
  • [9] Biochemical characterization of a novel cold-active, halophilic and organic solvent-tolerant lipase from B. licheniformis KM12 with potential application for biodiesel production
    Malekabadi, Saeid
    Badoei-dalfard, Arastoo
    Karami, Zahra
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 109 : 389 - 398
  • [10] Eco-Friendly Composite of Fe3O4-Reduced Graphene Oxide Particles for Efficient Enzyme Immobilization
    Patel, Sanjay K. S.
    Choi, Seung Ho
    Kang, Yun Chan
    Lee, Jung-Kul
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (03) : 2213 - 2222
123