Proteomic Characterization of Bradyrhizobium diazoefficiens Bacteroids Reveals a Post-Symbiotic, Hemibiotrophic-Like Lifestyle of the Bacteria within Senescing Soybean Nodules

被引：6

作者：

Strodtman, Kent N. ^{[1
]}

Frank, Sooyoung ^{[2
]}

Stevenson, Severin ^{[3
]}

Thelen, Jay J. ^{[2
]}

Emerich, David W. ^{[2
]}

机构：

[1] Columbia Coll, Dept Sci, Columbia, MO 65216 USA

[2] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA

[3] Charles River Labs, Carson City, NV 89523 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 12期

基金：

美国农业部;

关键词：

Bradyrhizobium diazoefficiens; soybean; Glycine max; nitrogen fixation; senescence; proteomics; hemibiotroph; POLY-BETA-HYDROXYBUTYRATE; JAPONICUM BACTEROIDS; NITROGEN-FIXATION; ROOT-NODULES; RHIZOBIUM; REDOX; SENESCENCE; ENZYMES; DIFFERENTIATION; METABOLISM;

D O I：

10.3390/ijms19123947

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The form and physiology of Bradyrhizobium diazoefficiens after the decline of symbiotic nitrogen fixation has been characterized. Proteomic analyses showed that post-symbiotic B. diazoefficiens underwent metabolic remodeling as well-defined groups of proteins declined, increased or remained unchanged from 56 to 119 days after planting, suggesting a transition to a hemibiotrophic-like lifestyle. Enzymatic analysis showed distinct patterns in both the cytoplasm and the periplasm. Similar to the bacteroid, the post-symbiotic bacteria rely on a non-citric acid cycle supply of succinate and, although viable, they did not demonstrate the ability to grow within the senescent nodule.

引用

页数：16