Primordial oscillations in life: Direct observation of glycolytic oscillations in individual HeLa cervical cancer cells

被引：23

作者：

Amemiya, Takashi ^{[1
]}

Shibata, Kenichi ^{[1
]}

Itoh, Yoshihiro ^{[1
]}

Itoh, Kiminori ^{[1
]}

Watanabe, Masatoshi ^{[2
]}

Yamaguchi, Tomohiko ^{[3
,4
]}

机构：

[1] Yokohama Natl Univ, Grad Sch Environm & Informat Sci, Hodogaya Ku, 79-7 Tokiwadai, Yokohama, Kanagawa 2408501, Japan

[2] Mie Univ, Grad Sch Med, 2-174 Edobashi, Tsu, Mie 5148507, Japan

[3] Natl Inst Adv Ind Sci & Technol, Res Inst Sustainable Chem, 1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan

[4] Meiji Inst Adv Study Math Sci MIMS, Nakano Ku, 4-21-1 Nakano, Tokyo 1648525, Japan

来源：

CHAOS | 2017年 / 27卷 / 10期

基金：

日本学术振兴会;

关键词：

ASCITES TUMOR-CELLS; CARBOHYDRATE-METABOLISM; GLUCOSE-METABOLISM; YEAST-CELLS; COMMUNICATION; DYNAMICS; SYNCHRONIZATION; STIMULATION; FIBROBLASTS; POPULATIONS;

D O I：

10.1063/1.4986865

中图分类号：

O29 [应用数学];

学科分类号：

070104 ;

摘要：

We report the first direct observation of glycolytic oscillations in HeLa cervical cancer cells, which we regard as primordial oscillations preserved in living cells. HeLa cells starved of glucose or both glucose and serum exhibited glycolytic oscillations in nicotinamide adenine dinucleotide (NADH), exhibiting asynchronous intercellular behaviors. Also found were spatially homogeneous and inhomogeneous intracellular NADH oscillations in the individual cells. Our results demonstrate that starved HeLa cells may be induced to exhibit glycolytic oscillations by either high-uptake of glucose or the enhancement of a glycolytic pathway (Crabtree effect or the Warburg effect), or both. Their asynchronous collective behaviors in the oscillations were probably due to a weak intercellular coupling. Elucidation of the relationship between the mechanism of glycolytic dynamics in cancer cells and their pathophysiological characteristics remains a challenge in future. Published by AIP Publishing.

引用

页数：8

共 43 条

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