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[1]
Habowski AN, 2020, COMMUN BIOL, V3, DOI 10.1038/s42003-020-01181-z
Transcriptomic and proteomic signatures of stemness and differentiation in the colon crypt (vol 3, 453, 2020)
被引：2
作者：
Habowski, Amber N.
Flesher, Jessica L.
Bates, Jennifer M.
Tsai, Chia-Feng
Martin, Kendall
Zhao, Rui
Ganesan, Anand K.
Edwards, Robert A.
Shi, Tujin
Wiley, H. Steven
Shi, Yongsheng
Hertel, Klemens J.
Waterman, Marian L.
机构：
[1] Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, 92697, CA
[2] Department of Biological Chemistry, University of California Irvine, Irvine, 92697, CA
[3] Institute for Immunology, University of California Irvine, Irvine, 92697, CA
[4] Biological Sciences Division, Pacific Northwest National Laboratory, Richland, 99354, WA
[5] Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, 99354, WA
[6] Department of Dermatology, University of California Irvine, Irvine, 92697, CA
[7] Department of Pathology and Laboratory Medicine, University of California Irvine, Irvine, 92697, CA
关键词：
D O I：
10.1038/s42003-020-01237-0
中图分类号：
Q [生物科学];
学科分类号：
07 ;
0710 ;
09 ;
摘要：
Intestinal stem cells are non-quiescent, dividing epithelial cells that rapidly differentiate into progenitor cells of the absorptive and secretory cell lineages. The kinetics of this process is rapid such that the epithelium is replaced weekly. To determine how the transcriptome and proteome keep pace with rapid differentiation, we developed a new cell sorting method to purify mouse colon epithelial cells. Here we show that alternative mRNA splicing and polyadenylation dominate changes in the transcriptome as stem cells differentiate into progenitors. In contrast, as progenitors differentiate into mature cell types, changes in mRNA levels dominate the transcriptome. RNA processing targets regulators of cell cycle, RNA, cell adhesion, SUMOylation, and Wnt and Notch signaling. Additionally, global proteome profiling detected >2,800 proteins and revealed RNA:protein patterns of abundance and correlation. Paired together, these data highlight new potentials for autocrine and feedback regulation and provide new insights into cell state transitions in the crypt. © 2020, The Author(s).
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