Endosomal sorting complexes for transport-III (ESCRT-III) assemble in vivo onto membranes with negative Gaussian curvature. How membrane shape influences ESCRT-III polymerization and how ESCRT-III shapes membranes is yet unclear. Human core ESCRT-III proteins, CHMP4B, CHMP2A, CHMP2B and CHMP3 are used to address this issue in vitro by combining membrane nanotube pulling experiments, cryo-electron tomography and AFM. We show that CHMP4B filaments preferentially bind to flat membranes or to tubes with positive mean curvature. Both CHMP2B and CHMP2A/CHMP3 assemble on positively curved membrane tubes. Combinations of CHMP4B/CHMP2B and CHMP4B/CHMP2A/CHMP3 are recruited to the neck of pulled membrane tubes and reshape vesicles into helical "corkscrew-like" membrane tubes. Sub-tomogram averaging reveals that the ESCRT-III filaments assemble parallel and locally perpendicular to the tube axis, highlighting the mechanical stresses imposed by ESCRT-III. Our results underline the versatile membrane remodeling activity of ESCRT-III that may be a general feature required for cellular membrane remodeling processes.
机构:
Univ Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Allison, Rachel
Lumb, Jennifer H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Lumb, Jennifer H.
论文数: 引用数:
h-index:
机构:
Fassier, Coralie
Connell, James W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Connell, James W.
Ten Martin, Daniel
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, UMR 7224, F-75252 Paris 05, France
INSERM, U952, F-75252 Paris 05, France
Univ Paris 06, F-75252 Paris 05, FranceUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Ten Martin, Daniel
Seaman, Matthew N. J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Clin Biochem, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Seaman, Matthew N. J.
Hazan, Jamile
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, UMR 7224, F-75252 Paris 05, France
INSERM, U952, F-75252 Paris 05, France
Univ Paris 06, F-75252 Paris 05, FranceUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Alonso Y Adell, Manuel
Migliano, Simona M.
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Migliano, Simona M.
Upadhyayula, Srigokul
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Pediat, Boston, MA 02115 USA
Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Upadhyayula, Srigokul
Bykov, Yury S.
论文数: 0引用数: 0
h-index: 0
机构:
European Mol Biol Lab, Struct & Computat Unit, Heidelberg, GermanyMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Bykov, Yury S.
论文数: 引用数:
h-index:
机构:
Sprenger, Simon
Pakdel, Mehrshad
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Max Planck Inst Biochem, Martinsried, GermanyMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Pakdel, Mehrshad
Vogel, Georg F.
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Med Univ Innsbruck, Div Histol & Embryol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Vogel, Georg F.
Jih, Gloria
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Jih, Gloria
Skillern, Wesley
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Skillern, Wesley
Behrouzi, Reza
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Behrouzi, Reza
论文数: 引用数:
h-index:
机构:
Babst, Markus
论文数: 引用数:
h-index:
机构:
Schmidt, Oliver
Hess, Michael W.
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Div Histol & Embryol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Hess, Michael W.
Briggs, John A. G.
论文数: 0引用数: 0
h-index: 0
机构:
European Mol Biol Lab, Struct & Computat Unit, Heidelberg, Germany
European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, GermanyMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Briggs, John A. G.
Kirchhausen, Tomas
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Pediat, Boston, MA 02115 USA
Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Kirchhausen, Tomas
论文数: 引用数:
h-index:
机构:
Teis, David
[J].
ELIFE,
2017,
6
[3]
Alqabandi M., 2019, PREPRINT, DOI [10.1101/756403v1, DOI 10.1101/756403V1]
机构:Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
Babst, M
Wendland, B
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
Wendland, B
Estepa, EJ
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
Estepa, EJ
Emr, SD
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USAUniv Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Belly, Agnes
Goldberg, Yves
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Goldberg, Yves
Chatellard-Causse, Christine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Chatellard-Causse, Christine
Blot, Beatrice
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Blot, Beatrice
Schoehn, Guy
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, EMBL, CNRS, UVHCI,UMI 3265, F-38042 Grenoble, France
CEA, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble, France
CNRS, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble, France
Univ Grenoble 1, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Cashikar, Anil G.
Shim, Soomin
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Shim, Soomin
Roth, Robyn
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Roth, Robyn
Maldazys, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Maldazys, Michael R.
Heuser, John E.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Heuser, John E.
Hanson, Phyllis I.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
机构:
Univ Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Allison, Rachel
Lumb, Jennifer H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Lumb, Jennifer H.
论文数: 引用数:
h-index:
机构:
Fassier, Coralie
Connell, James W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Connell, James W.
Ten Martin, Daniel
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, UMR 7224, F-75252 Paris 05, France
INSERM, U952, F-75252 Paris 05, France
Univ Paris 06, F-75252 Paris 05, FranceUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Ten Martin, Daniel
Seaman, Matthew N. J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cambridge, Dept Clin Biochem, Cambridge CB2 0XY, England
Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, EnglandUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
Seaman, Matthew N. J.
Hazan, Jamile
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, UMR 7224, F-75252 Paris 05, France
INSERM, U952, F-75252 Paris 05, France
Univ Paris 06, F-75252 Paris 05, FranceUniv Cambridge, Dept Med Genet, Cambridge CB2 0XY, England
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Alonso Y Adell, Manuel
Migliano, Simona M.
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Migliano, Simona M.
Upadhyayula, Srigokul
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Pediat, Boston, MA 02115 USA
Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Upadhyayula, Srigokul
Bykov, Yury S.
论文数: 0引用数: 0
h-index: 0
机构:
European Mol Biol Lab, Struct & Computat Unit, Heidelberg, GermanyMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Bykov, Yury S.
论文数: 引用数:
h-index:
机构:
Sprenger, Simon
Pakdel, Mehrshad
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Max Planck Inst Biochem, Martinsried, GermanyMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Pakdel, Mehrshad
Vogel, Georg F.
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Med Univ Innsbruck, Div Histol & Embryol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Vogel, Georg F.
Jih, Gloria
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Jih, Gloria
Skillern, Wesley
论文数: 0引用数: 0
h-index: 0
机构:
Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Skillern, Wesley
Behrouzi, Reza
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Behrouzi, Reza
论文数: 引用数:
h-index:
机构:
Babst, Markus
论文数: 引用数:
h-index:
机构:
Schmidt, Oliver
Hess, Michael W.
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Div Histol & Embryol, Innsbruck, AustriaMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Hess, Michael W.
Briggs, John A. G.
论文数: 0引用数: 0
h-index: 0
机构:
European Mol Biol Lab, Struct & Computat Unit, Heidelberg, Germany
European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, GermanyMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Briggs, John A. G.
Kirchhausen, Tomas
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Med Sch, Dept Pediat, Boston, MA 02115 USA
Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA 02115 USA
Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USAMed Univ Innsbruck, Bioctr, Div Cell Biol, Innsbruck, Austria
Kirchhausen, Tomas
论文数: 引用数:
h-index:
机构:
Teis, David
[J].
ELIFE,
2017,
6
[3]
Alqabandi M., 2019, PREPRINT, DOI [10.1101/756403v1, DOI 10.1101/756403V1]
机构:Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
Babst, M
Wendland, B
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
Wendland, B
Estepa, EJ
论文数: 0引用数: 0
h-index: 0
机构:Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
Estepa, EJ
Emr, SD
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USAUniv Calif San Diego, Div Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Belly, Agnes
Goldberg, Yves
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Goldberg, Yves
Chatellard-Causse, Christine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Chatellard-Causse, Christine
Blot, Beatrice
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
INSERM, Equipe 2, U836, F-38042 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
Blot, Beatrice
Schoehn, Guy
论文数: 0引用数: 0
h-index: 0
机构:
Univ Grenoble 1, EMBL, CNRS, UVHCI,UMI 3265, F-38042 Grenoble, France
CEA, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble, France
CNRS, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble, France
Univ Grenoble 1, Inst Biol Struct Jean Pierre Ebel, UMR 5075, F-38027 Grenoble, FranceUniv Grenoble 1, Grenoble Inst Neurosci, F-38042 Grenoble, France
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Cashikar, Anil G.
Shim, Soomin
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Shim, Soomin
Roth, Robyn
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Roth, Robyn
Maldazys, Michael R.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Maldazys, Michael R.
Heuser, John E.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Heuser, John E.
Hanson, Phyllis I.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA