Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women (vol 19, 188, 2020)

被引：1

作者：

Briand, Valerie ^{[1
,2
]}

Cottrell, Gilles ^{[2
]}

Tuikue Ndam, Nicaise ^{[2
]}

Martianez-Vendrell, Xavier ^{[3
]}

Vianou, Bertin ^{[4
]}

Mama, Atika ^{[4
]}

Kouwaye, Bienvenue ^{[4
]}

Houze, Sandrine ^{[2
,5
]}

Bailly, Justine ^{[5
]}

Gbaguidi, Erasme ^{[4
]}

Sossou, Darius ^{[4
]}

Massougbodji, Achille ^{[4
]}

Accrombessi, Manfred ^{[4
,6
]}

Mayor, Alfredo ^{[3
]}

Ding, Xavier C. ^{[7
]}

Fievet, Nadine ^{[2
]}

机构：

[1] Univ Bordeaux, Inst Rech Dev IRD, INSERM, UMR 1219, 146 Rue Leo Saignat, F-33076 Bordeaux, France

[2] Univ Paris, MERIT, IRD, F-75006 Paris, France

[3] Univ Barcelona, Hosp Clin Barcelona, ISGlobal, Barcelona 08036, Spain

[4] Inst Rech Clin Benin IRCB, Cotonou, Benin

[5] Hop Bichat Claude Bernard, Ctr Natl Reference Paludisme, AP HP, F-75017 Paris, France

[6] London Sch Hyg & Trop Med, Dis Control Dept, Fac Infect & Trop Dis, London WC1E 7HT, England

[7] FIND, CH-1202 Geneva, Switzerland

来源：

MALARIA JOURNAL | 2020年 / 19卷 / 01期

关键词：

Africa; Diagnostic tests; HRP-2; antigen; Malaria; Pregnancy;

D O I：

10.1186/s12936-020-03400-8

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: While sub-microscopic malarial infections are frequent and potentially deleterious during pregnancy, routine molecular detection is still not feasible. This study aimed to assess the performance of a Histidine Rich Protein 2 (HRP2)-based ultrasensitive rapid diagnostic test (uRDT, Alere Malaria Ag Pf) for the detection of infections of low parasite density in pregnant women. Methods: This was a retrospective study based on samples collected in Benin from 2014 to 2017. A total of 942 whole blood samples collected in 327 women in the 1st and 3rd trimesters and at delivery were tested by uRDT, conventional RDT (cRDT, SD BIOLINE Malaria Ag Pf), microscopy, quantitative polymerase chain-reaction (qPCR) and Luminex-based suspension array technology targeting P. falciparum HRP2. The performance of each RDT was evaluated using qPCR as reference standard. The association between infections detected by uRDT, but not by cRDT, with poor maternal and birth outcomes was assessed using multivariate regression models. Results: The overall positivity rate detected by cRDT, uRDT, and qPCR was 11.6% (109/942), 16.2% (153/942) and 18.3% (172/942), respectively. Out of 172 qPCR-positive samples, 68 were uRDT-negative. uRDT had a significantly better sensitivity (60.5% [52.7-67.8]) than cRDT (44.2% [36.6-51.9]) and a marginally decreased specificity (93.6% [91.7-95.3] versus 95.7% [94.0-97.0]). The gain in sensitivity was particularly high (33%) and statistically significant in the 1st trimester. Only 28 (41%) out of the 68 samples which were qPCR-positive, but uRDT-negative had detectable but very low levels of HRP2 (191 ng/mL). Infections that were detected by uRDT but not by cRDT were associated with a 3.4-times (95%CI 1.29-9.19) increased risk of anaemia during pregnancy. Conclusions: This study demonstrates the higher performance of uRDT, as compared to cRDTs, to detect low parasite density P. falciparum infections during pregnancy, particularly in the 1st trimester. uRDT allowed the detection of infections associated with maternal anaemia. © 2020 The Author(s).

引用

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