Multiwalled Carbon Nanotubes Modified by NTA-Copper Complex for Label-Free Electrochemical Immunosensor Detection

被引：17
作者：
Chebil, S. [1 ,2 ]
Macauley, N. [1 ,2 ,3 ]
Hianik, T. [3 ]
Korri-Youssoufi, H. [1 ,2 ]
机构：
[1] Univ Paris 11, CNRS UMR 8182, ICMMO, ECBB, F-91405 Orsay, France
[2] Univ Paris 11, F-91405 Orsay, France
[3] Comenius Univ, Fac Math Phys & Informat, Dept Nucl Phys & Biophys, Bratislava 84248, Slovakia
来源：
ELECTROANALYSIS | 2013年 / 25卷 / 03期
关键词：
Biosensors; Deep vein thrombosis; Label-free detection; MWCNT; NTA; copper; DIRECT ELECTRON-TRANSFER; D-DIMER; BINDING; BIOSENSORS; PROTEINS; AFFINITY;
D O I：
10.1002/elan.201200298
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A biomaterial based on multiwalled carbon nanotubes (MWCNTs) modified with His-tagged single chain D-dimer antibody trough nitrilo-triacetic-acid (NTA)/copper complex was designed for monitoring D-dimers, which were used as biomarkers of Deep Vein Thrombosis (DVT). The immunosensing evaluation of D-Dimer was followed by measuring the redox process of such complex. We demonstrate that using simple chemistry and exploiting the high surface area, electronic properties of MWCNTs and redox signal of the NTA/Cu2+ complex an efficient electrochemical immunosensor for the evaluation of wide range of D-dimer concentrations was obtained.
引用
收藏
页码：636 / 643
页数：8
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