3D high- and super-resolution imaging using single-objective SPIM

被引：0

作者：

Galland, Remi ^{[1
,2
,3
]}

Grenci, Gianluca ^{[3
]}

Aravind, Ajay ^{[3
]}

Viasnoff, Virgile ^{[3
,4
,5
]}

Studer, Vincent ^{[1
,2
]}

Sibarita, Jean-Baptiste ^{[1
,2
]}

机构：

[1] Univ Bordeaux, Interdisciplinary Inst Neurosci, Bordeaux, France

[2] CNRS, Bordeaux, France

[3] Natl Univ Singapore, Mechanobiol Inst, Singapore 117548, Singapore

[4] CNRS, Bio Mech Cellular Contacts, Singapore, Singapore

[5] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore

来源：

NATURE METHODS | 2015年 / 12卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; LOCALIZATION MICROSCOPY; PLANE ILLUMINATION; FLUORESCENT-PROBES; DIFFRACTION-LIMIT; LIVING CELLS; HIGH-DENSITY; MOLECULE; SILICON;

D O I：

10.1038/NMETH.3402

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.

引用

页码：641 / +

页数：9

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