Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats (vol 19, pg 26, 2017)

被引：0

作者：

Shan, Hai-Tao ^{[1
,2
]}

Zhang, Hai-Bo ^{[1
]}

Chen, Wen-Tao ^{[3
]}

Chen, Feng-Zhi ^{[1
]}

Wang, Tao ^{[4
]}

Luo, Jin-Tai ^{[1
]}

Yue, Min ^{[5
]}

Lin, Ji-Hong ^{[5
]}

Wei, An-Yang ^{[1
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Med Ctr Overseas Patients, Dept Urol, Guangzhou, Guangdong, Peoples R China

[2] Shawan Peoples Hosp, Dept Urol, Guangzhou, Guangdong, Peoples R China

[3] Shenzhen Hyde Med Equipment Co Ltd, Shenzhen, Peoples R China

[4] Longjiang Hosp, Dept Urol, Foshan, Peoples R China

[5] Southern Med Univ, Lab Anim Ctr, Guangzhou, Guangdong, Peoples R China

来源：

ASIAN JOURNAL OF ANDROLOGY | 2017年 / 19卷 / 01期

基金：

中国国家自然科学基金;

关键词：

bone marrow mesenchymal stem cell; diabetes mellitus; erectile function; low-energy shock-wave therapy;

D O I：

10.4103/1008-682X.189623

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Stem cell transplantation and low-energy shock-wave therapy (LESWT) have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups: the diabetic group (n = 6), the LESWT group (n = 6), the bone marrow-derived mesenchymal stem cell (BMSC) transplantation group (n = 15), and the combination of LESWT and BMSC transplantation group (n = 15). One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed: the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.

引用

页码：140 / 140

页数：1