Telomere-related prognostic biomarkers for survival assessments in pancreatic cancer

被引:15
作者
Chen, Shengyang [1 ]
Hu, Shuiquan [1 ]
Zhou, Baizhong [1 ]
Cheng, Bingbing [1 ]
Tong, Hao [1 ]
Su, Dongchao [1 ]
Li, Xiaoyong [1 ]
Chen, Yanjun [1 ]
Zhang, Genhao [2 ]
机构
[1] Zhengzhou Univ, Affiliated Hosp 5, Dept Hepatobiliary Pancreat Surg, Kangfu Front St 3, Zhengzhou 450052, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Blood Transfus, Zhengzhou, Peoples R China
关键词
WEB SERVER; EXPRESSION; GEMCITABINE; BREAST; CELLS;
D O I
10.1038/s41598-023-37836-0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human telomeres are linked to genetic instability and a higher risk of developing cancer. Therefore, to improve the dismal prognosis of pancreatic cancer patients, a thorough investigation of the association between telomere-related genes and pancreatic cancer is required. Combat from the R package "SVA" was performed to correct the batch effects between the TCGA-PAAD and GTEx datasets. After differentially expressed genes (DEGs) were assessed, we constructed a prognostic risk model through univariate Cox regression, LASSO-Cox regression, and multivariate Cox regression analysis. Data from the ICGC, GSE62452, GSE71729, and GSE78229 cohorts were used as test cohorts for validating the prognostic signature. The major impact of the signature on the tumor microenvironment and its response to immune checkpoint drugs was also evaluated. Finally, PAAD tissue microarrays were fabricated and immunohistochemistry was performed to explore the expression of this signature in clinical samples. After calculating 502 telomere-associated DEGs, we constructed a three-gene prognostic signature (DSG2, LDHA, and RACGAP1) that can be effectively applied to the prognostic classification of pancreatic cancer patients in multiple datasets, including TCGA, ICGC, GSE62452, GSE71729, and GSE78229 cohorts. In addition, we have screened a variety of tumor-sensitive drugs targeting this signature. Finally, we also found that protein levels of DSG2, LDHA, and RACGAP1 were upregulated in pancreatic cancer tissues compared to normal tissues by immunohistochemistry analysis. We established and validated a telomere gene-related prognostic signature for pancreatic cancer and confirmed the upregulation of DSG2, LDHA, and RACGAP1 expression in clinical samples, which may provide new ideas for individualized immunotherapy.
引用
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页数:12
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