Developing a Scalable Full Empty Separation Step for an AAV Capsid by Anion Exchange Chromatography in Step Gradient Elution Mode

被引：0

作者：

Staffler, Regina ^{[1
]}

Tsiaousi, Eleni ^{[1
]}

Weizenegger, Johanna ^{[1
]}

Babic, Jared ^{[1
]}

Alkharrat, Bilal ^{[2
]}

Weiss, Christina ^{[1
]}

Boscher, Matthias ^{[1
]}

Wagner, Johanna ^{[1
]}

Petrik, Sinisa ^{[1
]}

Ritter, Sebastian ^{[1
]}

Zach, Christine ^{[1
]}

Lott, Marie ^{[1
]}

Kloetzer, Tobias ^{[1
]}

Heinlein, Anita ^{[1
]}

Gora, Matthias ^{[1
]}

Youssef, Ahmed ^{[1
]}

Schoberth, Alexandra ^{[1
]}

Langhauser, Melanie ^{[1
]}

机构：

[1] Ascend GCTx, Martinsried, Germany

[2] Roche, Planegg, Germany

来源：

MOLECULAR THERAPY | 2023年 / 31卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

404

引用

页码：213 / 214

页数：2

共 35 条

[1] Developing an Anion Exchange Chromatography Assay for Determining Empty and Full Capsid Contents in AAV6.2
Wang, Chunlei
Mulagapati, Sri Hari Raju
Chen, Zhongying
Du, Jing
Zhao, Xiaohui
Xi, Guoling
Chen, Liyan
Linke, Thomas
Gao, Cuihua
Schmelzer, Albert E.
Liu, Dengfeng
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT, 2019, 15 : 257 - 263
[2] Anion-Exchange Chromatography at the Service of Gene Therapy: Baseline Separation of Full/Empty Adeno-Associated Virus Capsids by Screening of Conditions and Step Gradient Elution Mode
Aebischer, Megane K.
Gizardin-Fredon, Hugo
Lardeux, Honorine
Kochardt, Dominik
Elger, Carsten
Haindl, Markus
Ruppert, Raphael
Guillarme, Davy
D'Atri, Valentina
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (20)
[3] Separation of Full and Empty AAV Capsids by Anion Exchange
Murthy, Vidya
Mcwhirter, Asa Hagner
MOLECULAR THERAPY, 2022, 30 (04) : 239 - 239
[4] Empty-Full Capsid Separation of AAVS Using an Optimized Anion Exchange Chromatography Method
Schmudlach, Andrew
Yang, Hua
Koza, Stephan
Chen, Weibin
Lauber, Matthew
MOLECULAR THERAPY, 2020, 28 (04) : 380 - 380
[5] Separation of Empty Capsids from Full Capsids for AAV Gene Therapy Using a Flow through and Step Elution Approach
Arunkumar, Abhiram
Singh, Nripen
MOLECULAR THERAPY, 2021, 29 (04) : 119 - 119
[6] Adeno-associated viral capsid stability on anion exchange chromatography column and its impact on empty and full capsid separation
Khanal, Ohnmar
Kumar, Vijesh
Jin, Mi
MOLECULAR THERAPY METHODS & CLINICAL DEVELOPMENT, 2023, 31
[7] Use of anion exchange chromatography in weak partitioning mode to provide high empty AAV capsid removal and product yields
Davies, S.
Senaratne, J.
HUMAN GENE THERAPY, 2022, 33 (23-24) : A97 - A97
[8] Mass spectrometry friendly pH-gradient anion exchange chromatography for the separation of full and empty adeno-associated virus (AAV) capsids
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Fussl, Florian
Strasser, Lisa
Bones, Jonathan
ANALYTICAL METHODS, 2023, 15 (43) : 5788 - 5792
[9] Step Gradient Development for Full Particle Enrichment of AAV on Anion-Exchange Monoliths is Hindered by Inconsistent Column Lots
Raper, Austin T.
Reed, Andrew J.
Hartbarger, Sydney
Barber, Katy
Zhang, Jingjing
Oh, Younghoon
MOLECULAR THERAPY, 2023, 31 (04) : 221 - 221
[10] SEPARATION OF LOWER OXO ACIDS OF PHOSPHORUS BY GRADIENT ELUTION CHROMATOGRAPHY USING AN ANION EXCHANGE RESIN
KOGUCHI, K
WAKI, H
OHASHI, S
JOURNAL OF CHROMATOGRAPHY, 1966, 25 (02): : 398 - &

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