Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

被引:3
作者
Kumar, Neeraj [1 ,3 ]
Gismondi, Eric [2 ]
Reddy, Kotha Sammi [1 ]
机构
[1] ICAR Natl Inst Abiot Stress Management, Pune, India
[2] Univ Liege, Chem Inst, Freshwater & Ocean Sci Unit Res FOCUS, Lab Anim Ecol & Ecotoxicol LEAE, Liege, Belgium
[3] Natl Inst Abiot Stress Management, Pune 413115, Maharashtra, India
关键词
acute test; copper nanoparticles; fish; IBR; neurotransmitter; oxidative stress; CELLULAR STRESS PROTECTION; CARP CYPRINUS-CARPIO; FRESH-WATER TELEOST; RAINBOW-TROUT; OREOCHROMIS-MOSSAMBICUS; OXIDE NANOPARTICLES; OXIDATIVE STRESS; HEAT-SHOCK; ACETYLCHOLINESTERASE ACTIVITY; SELENIUM NANOPARTICLES;
D O I
10.1002/tox.24058
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu-NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50) for Cu and Cu-NPs were determined as 8.04 and 3.85 mg L-1, respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L-1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L-1 for Cu-NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione-s-transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu-NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu-NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu-NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu-NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu-NPs, induce toxicity and disrupt cellular metabolic activities in fish.
引用
收藏
页码:1581 / 1600
页数:20
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