Enhancement of immunogenicity and neutralizing responses against SARS-CoV-2 spike protein using the Fc fusion fragment

被引:5
作者
Ehteshaminia, Yahya [1 ]
Jalali, Seyedeh Farzaneh [2 ]
Jadidi-Niaragh, Farhad [3 ]
Enderami, Seyed Ehsan [5 ]
Pagheh, Abdol Sattar [4 ]
Akbari, Esmaeil [5 ]
Kenari, Saeid Abedian [5 ]
Hassannia, Hadi [5 ,6 ]
机构
[1] Univ Tehran Med Sci, Sch Publ Hlth, Dept Immunol, Tehran, Iran
[2] Kerman Univ Med Sci, Fac Allied Med, Dept Hematol, Kerman, Iran
[3] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran
[4] Birjand Univ Med Sci, Infect Dis Res Ctr, Birjand, Iran
[5] Mazandaran Univ Med Sci, Immunogenet Res Ctr, Sch Med, Sari, Iran
[6] Mazandaran Univ Med Sci, Sari 4815733971, Iran
关键词
COVID-19; vaccine; Spike protein; Fc fusion protein; SUBUNIT VACCINE; COVID-19; ANTIBODIES; RBD;
D O I
10.1016/j.lfs.2023.121525
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Aims: Vaccination has played an important role in protecting against death and the severity of COVID-19. The recombinant protein vaccine platform has a long track record of safety and efficacy. Here, we fused the SARS-CoV-2 spike S1 subunit to the Fc region of IgG and investigated immunogenicity, reactivity to human vacci-nated sera, and neutralizing activity as a candidate protein vaccine. Materials and method: We evaluated the immunogenicity of CHO-expressed S1-Fc fusion protein and tag-free S1 protein in rabbits via the production of S1-specific polyclonal antibodies. We subsequently compared the neutralizing activities of sera from immunized rabbits and human-vaccinated individuals using a surrogate Virus Neutralization Test (sVNT). Key findings: The results indicate that S1-specific polyclonal antibodies were induced in all groups; however, antibody levels were higher in rabbits immunized with S1-Fc fusion protein than tag-free S1 protein. Moreover, the reactivity of human vaccinated sera against S1-Fc fusion protein was significantly higher than tag-free S1 protein. Lastly, the results of the virus-neutralizing activity revealed that vaccination with S1-Fc fusion protein induced the highest level of neutralizing antibody response against SARS-CoV-2. Significance: Our results demonstrate that the S1 protein accompanied by the Fc fragment significantly enhances the immunogenicity and neutralizing responses against SARS-CoV-2. It is hoped that this platform can be used for human vaccination.
引用
收藏
页数:6
相关论文
共 45 条
[31]   Recombinant protein vaccines, a proven approach against coronavirus pandemics [J].
Pollet, Jeroen ;
Chen, Wen-Hsiang ;
Strych, Ulrich .
ADVANCED DRUG DELIVERY REVIEWS, 2021, 170 :71-82
[32]   Construction and immunogenic studies of a mFc fusion receptor binding domain (RBD) of spike protein as a subunit vaccine against SARS-CoV-2 infection [J].
Qi, Xiaoxiao ;
Ke, Bixia ;
Feng, Qian ;
Yang, Deying ;
Lian, Qinghai ;
Li, Zibo ;
Lu, Linlin ;
Ke, Changwen ;
Liu, Zhongqiu ;
Liao, Guochao .
CHEMICAL COMMUNICATIONS, 2020, 56 (61) :8683-8686
[33]   Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates [J].
Ren, Wenlin ;
Sun, Hunter ;
Gao, George F. ;
Chen, Jianxin ;
Sun, Sean ;
Zhao, Rongqing ;
Gao, Guang ;
Hu, Yalin ;
Zhao, Gan ;
Chen, Yuxin ;
Jin, Xia ;
Fang, Feng ;
Chen, Jinggong ;
Wang, Qi ;
Gong, Sitao ;
Gao, Wen ;
Sun, Yufei ;
Su, Junchi ;
He, Ailiang ;
Cheng, Xin ;
Li, Min ;
Xia, Chenxi ;
Li, Maohua ;
Sun, Le .
VACCINE, 2020, 38 (35) :5653-5658
[34]   SARS-CoV-2 Neutralizing Antibodies: A Network Meta-Analysis across Vaccines [J].
Rogliani, Paola ;
Chetta, Alfredo ;
Cazzola, Mario ;
Calzetta, Luigino .
VACCINES, 2021, 9 (03) :1-18
[35]   mRNA vaccines and clinical research in Africa-From hope to reality [J].
Saied, AbdulRahman A. .
INTERNATIONAL JOURNAL OF SURGERY, 2022, 105
[36]  
Shrotri M, 2022, LANCET HEALTH LONGEV, V3, pE470, DOI 10.1016/S2666-7568(22)00147-7
[37]   DNA vaccines against COVID-19: Perspectives and challenges [J].
Silveira, Marcelle Moura ;
Schmidt Garcia Moreira, Gustavo Marcal ;
Mendonca, Marcelo .
LIFE SCIENCES, 2021, 267
[38]   COVID-19 phase 4 vaccine candidates, effectiveness on SARS-CoV-2 variants, neutralizing antibody, rare side effects, traditional and nano-based vaccine platforms: a review [J].
Simnani, Faizan Zarreen ;
Singh, Dibyangshee ;
Kaur, Ramneet .
3 BIOTECH, 2022, 12 (01)
[39]  
Sun S., 2020, BIORXIV, DOI [10.1101/2020.11.29.402339, DOI 10.1101/2020.11.29.402339]
[40]   Comparison of Neutralizing Antibody Responses at 6 Months Post Vaccination with BNT162b2 and AZD1222 [J].
Terpos, Evangelos ;
Karalis, Vangelis ;
Ntanasis-Stathopoulos, Ioannis ;
Evangelakou, Zoi ;
Gavriatopoulou, Maria ;
Manola, Maria S. ;
Malandrakis, Panagiotis ;
Gianniou, Despoina D. ;
Kastritis, Efstathios ;
Trougakos, Ioannis P. ;
Dimopoulos, Meletios A. .
BIOMEDICINES, 2022, 10 (02)