Pyrolysis temperature affects biochar suitability as an alternative rhizobial carrier

Biochars produced from different feedstocks and at different pyrolysis temperatures may have various chemical and physical properties, affecting their potential use as alternative microbial carrier materials. In this study, biochars were produced from pine wood and oak feedstocks at various temperatures (400 degrees C, 500 degrees C, 600 degrees C, 700 degrees C and 800 degrees C), characterized, and assessed for their potential as carriers for Bradyrhizobium japonicum (CB1809) strain. The biochars were then stored at two different storage temperatures (28 degrees C and 38 degrees C) for up to 90 days. Furthermore, the study also explored the role of potentially ideal carriers as inoculants in the growth of Glycine max L. (soybean) under different moisture levels i.e., 55% water holding capacity (WHC) (D0), 30% WHC (D1) and, 15% WHC (D2) using a mixture of 50% garden soil and 50% sand. The results were compared to a control group (without inoculants) and a peat inoculant. Among all the materials derived from pine wood and oak, pine wood biochar pyrolyzed at 400degree celsius (P-BC400) exhibited the highest CFU count, with values of 10.34 and 9.74 Log 10 CFU g(- 1) after 90 days of storage at 28degree celsius and 38degree celsius, respectively. This was notably higher compared to other biochars and peat carriers. Significant (p < 0.05) increases in plant properties: shoot and root dry biomass (174% and 367%), shoot and root length (89% and 85%), number of leaves (71%), membrane stability index (27%), relative water content (26%), and total chlorophyll (140%) were observed in plants treated with P-BC400 carrier inoculant compared to the control at D2; however, lower enrichment of delta C-13 (37%) and delta N-15 (108%) with highest number of root nodules (8.3 +/- 1.26) and nitrogenase activity (0.869 +/- 0.04) were observed under D2, as evident through PCA analysis, showing more nitrogen (N) fixation and photosynthetic activity. Overall, this experiment concluded that biochar pyrolyzed at lower temperatures, especially P-BC400, was the most suitable candidate for rhizobial inoculum and promoted soybean growth.