Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

被引:4
作者
Postnikov, Eugene B. [1 ]
Wasiak, Michal [2 ]
Bartoszek, Mariola [3 ]
Polak, Justyna [3 ]
Zyubin, Andrey [4 ]
Lavrova, Anastasia I. [4 ,5 ]
Chorazewski, Miroslaw [3 ]
机构
[1] Kursk State Univ, Theoret Phys Dept, Radishcheva St 33, Kursk 305000, Russia
[2] Univ Lodz, Dept Phys Chem, Ul Pomorska 165, PL-90236 Lodz, Poland
[3] Univ Silesia Katowice, Inst Chem, Ul Szkolna 9, PL-40006 Katowice, Poland
[4] Immanuel Kant Balt Fed Univ, Sophya Kovalevskaya North West Math Res Ctr, Nevskogo St 14, Kaliningrad 236041, Russia
[5] St Petersburg State Res Inst Phthisiopulmonol, Ligovskiy Prospect 2-4, St Petersburg 194064, Russia
关键词
cell wall; Mucobacterium sp; antioxidants; EPR; Raman; DSC; MYCOBACTERIUM-TUBERCULOSIS INFECTION; MANNOSE-CAPPED LIPOARABINOMANNAN; RADICAL-SCAVENGING ACTIVITIES; OXIDATIVE STRESS; ANTIOXIDANT PROPERTIES; EPR SPECTROSCOPY; BETA-CAROTENE; ANTIRADICAL PROPERTIES; NONINVASIVE DETECTION; SUPEROXIDE-DISMUTASE;
D O I
10.3390/molecules28176417
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this work, we review some physical methods of macroscopic experiments, which have been recently argued to be promising for the acquisition of valuable characteristics of biomolecular structures and interactions. The methods we focused on are electron paramagnetic resonance spectroscopy, Raman spectroscopy, and differential scanning calorimetry. They were chosen since it can be shown that they are able to provide a mutually complementary picture of the composition of cellular envelopes (with special attention paid to mycobacteria), transitions between their molecular patterning, and the response to biologically active substances (reactive oxygen species and their antagonists-antioxidants-as considered in our case study).
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页数:27
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