Trypsin/α-Amylase Inhibitors from Capsicum chinense Seeds: Characterization and Antifungal Activity against Fungi of Agronomic Importance

被引:3
作者
da Silva, Marciele Souza [1 ]
dos Santos, Layrana de Azevedo [1 ]
Taveira, Gabriel Bonan [1 ]
Nagano, Celso Shiniti [2 ]
Chaves, Renata Pinheiro [2 ]
Carvalho, Andre de Oliveira [1 ]
Rodrigues, Rosana [3 ]
Gomes, Valdirene Moreira [1 ]
机构
[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Fisiol & Bioquim Microrganismos, Campos Dos Goytacazes, RJ, Brazil
[2] Univ Fed Ceara UFC, Dept Engn Pesca, BioMar Lab, Lab Bioquim Marinha, Fortaleza, Ceara, Brazil
[3] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, Lab Melhoramento & Genet Vegetal, Campos Dos Goytacazes, RJ, Brazil
关键词
Antimicrobial peptides; alpha-amylase inhibitor; Capsicum; phytopathogenic fungi; plant defense; protease inhibitors; ALPHA-AMYLASE/TRYPSIN; PROTEINASE-INHIBITORS; ANNUUM FRUITS; PEPTIDES; IDENTIFICATION; PURIFICATION; RESISTANCE; PERMEABILIZATION; MECHANISM; PROTEASES;
D O I
10.2174/0929866530666230221141804
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Protease inhibitors (PIs) have attracted attention due to their important roles in plant defense. Objective: The objective of this work was to characterize and evaluate the antimicrobial activity of the peptides of a family of serine PIs from Capsicum chinense Jacq. seeds. Methods: Initially, PIs were extracted from the seeds and subjected to purification by chromatography, resulting in three different peptide enriched fractions (PEFs) termed PEF1, PEF2 and PEF3. Subsequently, the PEF3 was subjected to trypsin inhibition assays, a- amylase activity assays, antimicrobial activity assays on phytopathogenic fungi, and assays to determine the likely mechanisms of action. Results: The PEF3 was composed of three protein bands with molecular masses ranging between 6 and 14 kDa. The amino acid residues of the similar to 6 kDa band showed high similarity with serine PIs. PEF3 inhibited the activity of the enzymes trypsin, human salivary a-amylase, and Tenebrio molitor larval a-amylase and inhibited the growth of phytopathogenic fungi, showing 83.7% loss of viability in Fusarium oxysporum. PEF3 induced reactive oxygen species in Colletotrichum lindemuthianum and F. oxysporum to dissipate their mitochondrial membrane potential and activated caspases in C. lindemuthianum. Conclusion: Our results reinforce the importance of PIs in plant defense mechanisms against phytopathogenic fungi as well as in their biotechnological applications for the control of plant pathogens.
引用
收藏
页码:260 / 274
页数:15
相关论文
共 69 条
[1]   Antifungal Mechanism of Action of Lactoferrin: Identification of H+-ATPase (P3A-Type) as a New Apoptotic-Cell Membrane Receptor [J].
Andres, Maria T. ;
Acosta-Zaldviar, Maikel ;
Fierro, Jose F. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2016, 60 (07) :4206-4216
[2]   Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages [J].
Baba, Viviane Y. ;
Powell, Adrian F. ;
Ivamoto-Suzuki, Suzana T. ;
Pereira, Luiz F. P. ;
Vanzela, Andre L. L. ;
Giacomin, Renata M. ;
Strickler, Susan R. ;
Mueller, Lukas A. ;
Rodrigues, Rosana ;
Goncalves, Leandro S. A. .
SCIENTIFIC REPORTS, 2020, 10 (01)
[3]   Antifungal and antimicrobial proteins and peptides of potato (Solanum tuberosum L.) tubers and their applications [J].
Bartova, Veronika ;
Barta, Jan ;
Jarosova, Marketa .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (14) :5533-5547
[4]   Isolation, characterization and mechanism of action of an antimicrobial peptide from Lecythis pisonis seeds with inhibitory activity against Candida albicans [J].
Brambila Vieira, Maria Eliza ;
Vasconcelos, Ilka Maria ;
Tavares Machado, Olga Lima ;
Gomes, Valdirene Moreira ;
Carvalho, Andre de Oliveira .
ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2015, 47 (09) :716-729
[5]   Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases [J].
Camo, Cristina ;
Bonaterra, Anna ;
Badosa, Esther ;
Baro, Aina ;
Montesinos, Laura ;
Montesinos, Emilio ;
Planas, Marta ;
Feliu, Lidia .
PEPTIDES, 2019, 112 :85-95
[6]   A structural perspective of plant antimicrobial peptides [J].
Campos, Marcelo Lattarulo ;
Liao, Luciano Morais ;
Fernandes Alves, Eliane Santana ;
Migliolo, Ludovico ;
Dias, Simoni Campos ;
Franco, Octavio Luiz .
BIOCHEMICAL JOURNAL, 2018, 475 :3359-3375
[7]   H-3, a new lectin from the marine sponge Haliclona caerulea: Purification and mass spectrometric characterization [J].
Carneiro, Romulo Farias ;
de Melo, Arthur Alves ;
de Almeida, Alexandra Sampaio ;
Moura, Raniere da Mata ;
Chaves, Renata Pinheiro ;
de Sousa, Bruno Lopes ;
do Nascimento, Kyria Santiago ;
Sampaio, Silvana Saker ;
Matos Santos Lima, Joao Paulo ;
Cavada, Benildo Sousa ;
Nagano, Celso Shiniti ;
Sampaio, Alexandre Holanda .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2013, 45 (12) :2864-2873
[8]  
Carrizo Garcia C, 2013, BREAKTHROUGHS GENETI, P205
[9]   Phylogenetic relationships, diversification and expansion of chili peppers (Capsicum, Solanaceae) [J].
Carrizo Garcia, Carolina ;
Barfuss, Michael H. J. ;
Sehr, Eva M. ;
Barboza, Gloria E. ;
Samuel, Rosabelle ;
Moscone, Eduardo A. ;
Ehrendorfer, Friedrich .
ANNALS OF BOTANY, 2016, 118 (01) :35-51
[10]   Plant Serine Protease Inhibitors: Biotechnology Application in Agriculture and Molecular Farming [J].
Clemente, Marina ;
Corigliano, Mariana G. ;
Pariani, Sebastian A. ;
Sanchez-Lopez, Edwin F. ;
Sander, Valeria A. ;
Ramos-Duarte, Victor A. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (06)