Characterization of the synergistic inhibitory effect of cyanidin-3-O-glucoside and catechin on pancreatic lipase

被引:26
作者
Wang, Yuying [1 ,2 ]
Chen, Lihang [1 ,2 ]
Liu, Huimin [1 ,2 ]
Xie, Jiahan [1 ,2 ]
Yin, Wandi [1 ,2 ]
Xu, Zhenghang [1 ]
Ma, Huijing [1 ]
Wu, Wenzhuo [1 ]
Zheng, Mingzhu [1 ,2 ]
Liu, Meihong [1 ,2 ]
Liu, Jingsheng [1 ,2 ]
机构
[1] Jilin Agr Univ, Coll Food Sci & Engn, Changchun 130118, Jilin, Peoples R China
[2] Natl Engn Res Ctr Wheat & Corn Deep Proc, Changchun 130118, Jilin, Peoples R China
关键词
Ultrafiltration; Cyanidin-3-O-glucoside; Catechin; Synergistic effect; Pancreatic lipase; Molecular docking; ANTHOCYANINS; EXTRACTS; POLYPHENOLS; PROANTHOCYANIDINS; SUBFRACTIONS;
D O I
10.1016/j.foodchem.2022.134672
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This study aimed to identify novel pancreatic lipase (PL) inhibitors using affinity ultrafiltration combined with spectroscopy and molecular docking. Cyanidin-3-O-glucoside (C3G; IC50: 0.268 mg/mL) and catechin (IC50: 0.280 mg/mL) were shown to be potent PL inhibitors extracted from black rice and adzuki bean coat extracts. Isobologram analysis revealed that the combined use of C3G and catechin at a ratio of 2:3 had a remarkable synergistic effect (IC50 of the mixture: 0.201 mg/mL). The inhibitory mechanism of C3G-catechin mixture was of mixed type. The C3G-catechin mixture had a great impact on PL secondary structures. Molecular docking analysis further demonstrated that these polyphenols formed hydrophobic interactions and hydrogen bonds with amino acid residues in the binding pocket of PL. Collectively, C3G and catechin were shown to inhibit PL in a synergistic manner and can be potentially used for the development of food supplements for obesity prevention.
引用
收藏
页数:9
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