Full-length transcriptome sequencing of lymphocytes respond to IFN-γ reveals a Th1-skewed immune response in flounder (Paralichthys olivaceus)

被引:5
|
作者
He, Ziyang [1 ]
Tian, Hongfei [1 ]
Xing, Jing [1 ,2 ,3 ]
Tang, Xiaoqian [1 ]
Sheng, Xiuzhen [1 ]
Chi, Heng [1 ]
Zhan, Wenbin [1 ,2 ]
机构
[1] Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
[3] Ocean Univ China, Fisheries Coll, Lab Pathol & Immunol Aquat Anim, Qingdao 266003, Peoples R China
基金
中国国家自然科学基金;
关键词
IFN-; CD4+T cells; T-bet; Th1 cell differentiation; Paralichthys olivaceus; TROUT ONCORHYNCHUS-MYKISS; EXPRESSION ANALYSIS; JAPANESE FLOUNDER; T-CELLS; IDENTIFICATION; ADJUVANT; GENES; CD4-2; BET;
D O I
10.1016/j.fsi.2023.108636
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Interferon gamma (IFN-gamma), the member of type II interferons, is a major driver and effector cytokine for Th1 cells and plays broad roles in regulating the function of immune cells. Teleost fish represents the oldest living bony vertebrates containing T-lymphocyte subsets. However, whether or how the regulatory mechanisms of IFN-gamma on Th1 cells occur in teleost fish remain unknown. In this study, full-length transcriptome sequencing was per-formed to analyze the differentially expressed genes (DEGs) and signaling pathways in the IFN-gamma stimulated lymphocytes of flounder (Paralichthys olivaceus), the data showed 811 genes were upregulated and 1107 genes were downregulated, Th1 and Th2 cell differentiation pathway was remarkably enriched from DEGs, and the genes in the Th1 cell differentiation pathway were upregulated and verified. Accordingly, variations on Th1 cell differentiation marker genes and CD4+ cells were investigated after IFN-gamma stimulation, the results confirmed that CD4+ T lymphocytes proliferated significantly after IFN-gamma stimulation, accompanied by eight genes significant upregulation and increased T-bet expression in lymphocytes. In conclusion, the results revealed an induction of IFN-gamma on Th1-type immune response, providing novel perspectives into the differentiation of CD4+ T lympho-cytes in teleost.
引用
收藏
页数:10
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