Genotypes and virulence-related activities of Candida albicans derived from oral cavity of patients in Hokkaido

被引：2

作者：

Ouchi, Chisato ^{[1
,2
,3
]}

Hasebe, Akira ^{[2
,3
,5
,6
]}

Sakata, Ken-ichiro ^{[1
]}

Sato, Jun ^{[1
]}

Yamazaki, Yutaka ^{[4
]}

Ohga, Noritaka ^{[1
]}

Kitagawa, Yoshimasa ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Dent Med, Oral Diag & Med, Sapporo, Japan

[2] Hokkaido Univ, Fac Dent Med, Dept Oral Mol Microbiol, Sapporo, Japan

[3] Hokkaido Univ, Grad Sch Dent Med, Sapporo, Japan

[4] Hokkaido Univ, Fac Dent Med, Dept Oral Hlth Sci, Gerodontol, Sapporo, Japan

[5] Hokkaido Univ, Fac Dent Med, Dept Oral Mol Microbiol, Kita 13,Nishi 7,Kita Ku, Sapporo 0608586, Japan

[6] Hokkaido Univ, Grad Sch Dent Med, Kita 13,Nishi 7,Kita Ku, Sapporo 0608586, Japan

来源：

ARCHIVES OF ORAL BIOLOGY | 2024年 / 157卷

基金：

日本学术振兴会;

关键词：

Candida albicans; Oral candidiasis; Pathogenesis; Genotype; PATHOGENICITY; EPIDEMIOLOGY; EXPRESSION; GLABRATA; BIOFILM;

D O I：

10.1016/j.archoralbio.2023.105827

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: This study aimed to elucidate the difference in virulence of Candida albicans derived from oral candidiasis and non-oral candidiasis patients, and its genotype differences in Hokkaido to obtain a clue of a platform to develop new approaches for diagnosis and treatment.Design: C. albicans strains were collected from patients who visited the Hokkaido University Hospital Dental Center. Each strain was examined to i) identify the Candida albicans genotype by PCR, ii) measure the strain's extracellular secretory enzyme activity, iii) determine the strain's ability to induce the production of interleukin-8, and iv) determine the strain's ability to induce cell death.Results: Certain virulence-related protease activities and cytotoxicity were higher in strains derived from patients with oral candidiasis compared with strains derived from patients without oral candidiasis. This is the first report on genotypes and the virulence-related activities, such as some protease secretion, IL-8 induction and cytotox-icity of C. albicans in Hokkaido.Conclusions: The virulence-related activities of the fungal strain may influence the pathogenesis of oral candi-diasis, such as production of secreted aspartyl protease and cytotoxicity. In addition, C. albicans genotype C may be important for pathogenicity in Hokkaido, because the ratio of genotype C was increased in strains derived from oral candidiasis patients.

引用

页数：11

共 37 条

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