Bioprospecting Microalgae from Sewage Water: Assessment of Biochemicals for Biomass Utilization

Microalgal species from sewage treatment plant were identified by 18S rRNA sequencing and were explored for total lipids, carbohydrate, and protein contents, to serve as a potential candidate for biorefinery. Seven unicellular microalgae were identified as Chlorella sorokiniana, Dictyosphaerium sp., Graesiella emersonii belonging to Chlorellaceae and Scenedesmus sp., Desmodesmus sp., Tetranephris brasiliensis, and Coelastrella sp. belonging to Scenedesmaceae family. Biochemical assessment of all isolates revealed total lipid content from 17.49 +/- 1.41 to 47.35 +/- 0.61% w/w, total carbohydrate content from 12.82 +/- 0.19 to 64.29 +/- 0.63% w/w, and total protein content from 8.55 +/- 0.19 to 16.65 +/- 0.20% w/w. FAME analysis of extracted lipid was found to be rich in Hexadecane (C16:0), Tetradecane (C17:0), Octadecane (C18:0), Eicosane (C20:0), Tetracosane (C24:0), Pentacosane (C25:0) fatty acids, the presence of which makes excellent candidate for biodiesel. Being rich in lipid, microalgae Chlorella sorokiniana, Coelastrella sp., and Scenedesmus sp. have high potential for biofuels. Due to the presence of high protein content, Scenedesmus sp. and Chlorella sorokiniana can serve as food or feed supplement, whereas the high carbohydrate content of Dictyosphaerium sp., Coelastrella sp., and Scenedesmus sp. makes them an ideal candidate for fermentative production of alcohol and organic acids. Chlorella sp. and Scenedesmus sp., being dominant microalgae across all seasons, demonstrate remarkable resilience for their cultivation in sewage water and utilization of biomass in biorefineries.