A Bio-Inspired Free-Standing Film with Versatility: From Heterogeneous CTCs Programed Isolating to Breast Cancer Molecular Typing

被引：67

作者：

Bai, Mengqi ^{[1
]}

Qin, Rong ^{[2
]}

Cheng, Kai ^{[3
]}

Wang, Zengkai ^{[1
]}

Zhang, Liwei ^{[1
]}

Tian, Xiaohua ^{[4
]}

Zhang, Feiyi ^{[1
]}

Yang, Yuhe ^{[1
]}

Hou, Shuai ^{[1
]}

Xu, Jian ^{[5
]}

Liu, Lei ^{[1
]}

机构：

[1] Jiangsu Univ, Inst Adv Mat, Sch Mat Sci & Engn, Zhenjiang 212013, Peoples R China

[2] Jiangsu Univ, Nanjing Med Univ, Zhenjiang Clin Med Coll, Dept Med Oncol,Affiliated Peoples Hosp, Zhenjiang 212000, Peoples R China

[3] Yangzhou Univ, Coll Food Sci & Engn, Yangzhou 225009, Peoples R China

[4] Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China

[5] Nanjing Med Univ, Affiliated Hosp 1, Dept Lab Med, Nanjing 210029, Peoples R China

来源：

ADVANCED MATERIALS | 2024年 / 36卷 / 09期

基金：

中国国家自然科学基金;

关键词：

bio-inspired free-standing film; cancer molecular typing; dynamic bioactive biointerface; heterogeneous CTCs; programmed isolation; CIRCULATING TUMOR-CELLS; PEPTIDE; DYNAMICS; CAPTURE;

D O I：

10.1002/adma.202309046

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Developing a robust strategy for profiling heterogeneous circular tumor cells specifically, distinguishing the phenotypes of which in blood sample of cancer patient precisely, and releasing them sequentially, is significant for cancer management by liquid biopsy. Herein, a bio-inspired free-standing and flexible film composed of TiO2 nanotube and silk fibroin, fabricated with multiply dynamic bioactive surface (TSF/MDBS) by a simple and eco-friendly way including using polydopamine chemistry and dual dynamic covalent chemistry, is reported. The as-prepared TSF/MDBS binds specific peptides toward cells with epithelial biomarker and human epithelial growth factor receptor 2 (HER2) biomarker, and antifouling agents bovine serum albumin for obviating platelets and proteins adhering of blood, can capture heterogeneous CTCs with enhanced capability due to the cytocompatible soft film and exquisite surface design, and further release the captured cells as program, by specifically breaking down the covalent bonds in sequence via the action of adding biocompatible molecules fructose and glutathione. By applying the TSF/MDBS, it can be tailored into desired pieces for identifying CTCs with different phenotypes (HER2-high and HER2-low) from the unprocessed blood samples of breast cancer patients, and finally profiling these heterogeneous CTCs, to discriminate HER2 positive or negative of breast cancer patients in clinical applications. A free-standing film composed of titanium dioxide nanotube/silk fibroin is developed by an eco-friendly way. It is an easy-to-use platform for capturing and profiling heterogeneous circular tumor cells (CTCs) effectively and accurately from the unprocessed blood samples of patients, discriminating the CTCs with different surface biomarkers, showing the potential in molecular typing of breast cancer.image

引用

页数：12

共 36 条

[11] Stimulating Efficient and Stable Ultralong Phosphorescence of 2D Perovskites by Dual-Mode Triplet Exciton Stabilization [J].

Ge, Lei ;

Wang, Yang ;

Gao, Xiang ;

Li, Min ;

Zhang, Runqi ;

Liu, Siyu ;

Yu, Yihang ;

Li, Mingguang ;

Xu, Ligang ;

Tao, Ye ;

Chen, Runfeng ;

Lv, Wenzhen .

CHEMISTRY OF MATERIALS, 2022, 34 (19) :8917-8924

[12] Phenotypic Profiling of Circulating Tumor Cells in Metastatic Prostate Cancer Patients Using Nanoparticle-Mediated Ranking [J].

Green, Brenda J. ;

Nguyen, Vivian ;

Atenafu, Eshetu ;

Weeber, Phillip ;

Duong, Bill T. V. ;

Thiagalingam, Punithan ;

Labib, Mahmoud ;

Mohamadi, Reza M. ;

Hansen, Aaron R. ;

Joshua, Anthony M. ;

Kelley, Shana O. .

ANALYTICAL CHEMISTRY, 2019, 91 (15) :9348-9355

[13] Multifunctional "Smart" Particles Engineered from Live Immunocytes: Toward Capture and Release of Cancer Cells [J].

Huang, Chao ;

Yang, Gao ;

Ha, Qing ;

Meng, Jinxin ;

Wang, Shutao .

ADVANCED MATERIALS, 2015, 27 (02) :310-313

[14] Inspiration and application in the evolution of biomaterials [J].

Huebsch, Nathaniel ;

Mooney, David J. .

NATURE, 2009, 462 (7272) :426-432

[15] Isolation and Analysis of Tumor Cell Subpopulations Using Biomimetic Immuno-Fluorescent Magnetic Multifunctional Nanoprobes [J].

Li, Qingjin ;

Liao, Ziyi ;

Han, Lan ;

Li, Linyao ;

Song, Yang ;

Song, Erqun .

ADVANCED FUNCTIONAL MATERIALS, 2020, 30 (49)

[16] Circulating tumor cells: biology and clinical significance [J].

Lin, Danfeng ;

Shen, Lesang ;

Luo, Meng ;

Zhang, Kun ;

Li, Jinfan ;

Yang, Qi ;

Zhu, Fangfang ;

Zhou, Dan ;

Zheng, Shu ;

Chen, Yiding ;

Zhou, Jiaojiao .

SIGNAL TRANSDUCTION AND TARGETED THERAPY, 2021, 6 (01)

[17] Fabrication of aptamer modified TiO2 nanofibers for specific capture of circulating tumor cells [J].

Liu, Hui ;

Sun, Na ;

Ding, Pi ;

Chen, Changchong ;

Wu, Zeen ;

Zhu, Weipei ;

Liu, Lifen ;

Wang, Zhili ;

Pei, Renjun .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2020, 191

[18] A Versatile Dynamic Mussel-Inspired Biointerface: From Specific Cell Behavior Modulation to Selective Cell Isolation [J].

Liu, Lei ;

Tian, Xiaohua ;

Ma, Yue ;

Duan, Yuqing ;

Zhao, Xin ;

Pan, Guoqing .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (26) :7878-7882

[19] Phage-Based Profiling of Rare Single Cells Using Nanoparticle-Directed Capture [J].

Ma, Yuan ;

Chen, Kangfu ;

Xia, Fan ;

Atwal, Randy ;

Wang, Hansen ;

Ahmed, Sharif U. ;

Cardarelli, Lia ;

Lui, Irene ;

Duong, Bill ;

Wang, Zongjie ;

Wells, James A. ;

Sidhu, Sachdev S. ;

Kelley, Shana O. .

ACS NANO, 2021, 15 (12) :19202-19210

[20] CTCs in early breast cancer: A path worth taking [J].

Maltoni, Roberta ;

Gallerani, Giulia ;

Fici, Pietro ;

Rocca, Andrea ;

Fabbri, Francesco .

CANCER LETTERS, 2016, 376 (02) :205-210

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