Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids

被引：9

作者：

Johnson, Coreen ^{[1
,2
]}

Marquez, Christopher ^{[4
]}

Olson, Damon ^{[5
]}

Ward, Tabitha ^{[2
]}

Cheney, Stephen ^{[2
]}

Hulten, Tina ^{[3
]}

Ton, Trang ^{[1
]}

Webb, C. R. ^{[1
]}

Dunn, James ^{[1
,2
]}

机构：

[1] Texas Childrens Hosp, Dept Pathol, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Pathol & Immunol, Houston, TX 77030 USA

[3] Baylor Coll Med, Sect Infect Dis, Dept Pediat, Houston, TX 77030 USA

[4] Mayo Clin, Dept Lab Med & Pathol, Jacksonville, FL 32224 USA

[5] Childrens Minnesota, Dept Pathol, Minneapolis, MN 55404 USA

来源：

FUTURE MICROBIOLOGY | 2023年 / 18卷 / 03期

关键词：

cerebrospinal fluid; Kingella kingae; multiplex PCR; osteoarticular infection; pleural fluid; synovial fluid; POLYMERASE-CHAIN-REACTION; KINGELLA-KINGAE; SEPTIC ARTHRITIS; OSTEOARTICULAR INFECTIONS; DIAGNOSIS; CHILDREN; SEROTYPES;

D O I：

10.2217/fmb-2022-0226

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: To assess the performance characteristics of a lab-developed multiplex PCR assay for the detection of common bacterial pathogens associated with infections in pediatric patients from normally sterile sites, such as cerebrospinal fluid, synovial and pleural fluids. Materials & methods: A total of 272 specimens were tested by PCR and traditional culture methods to assess the presence of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, methicillin-sensitive and methicillin-resistant Staphylococcus aureus, and Kingella kingae. Results: Compared with culture, the overall positive and negative percentage agreement of the PCR were 95.9% and 74.1%, respectively. Conclusion: This sterile body fluid PCR affords a rapid and sensitive alternative for bacterial detection, allowing for more timely pathogen-directed antimicrobial therapy.

引用

页码：187 / 195

页数：9

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