A triphenylamine-based fluorescence probe for detection of hypochlorite in mitochondria

被引：24

作者：

Yu, Guan-Hua ^{[1
]}

Hu, Hao-Ran ^{[1
]}

Liu, Rui-Bin ^{[2
]}

Sheng, Guo-Zhu ^{[2
]}

Niu, Jia-Jie ^{[2
]}

Fang, Ying ^{[1
]}

Wang, Kun-Peng ^{[1
]}

Hu, Zhi-Qiang ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, MOE, Qingdao 266042, Peoples R China

[2] Shandong Kangqiao Biotechnol Co Ltd, Binzhou 256500, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2023年 / 299卷

基金：

中国国家自然科学基金;

关键词：

Triphenylamine; Fluorescence probe; ClO-detection; Mitochondrial targeting; HCLO; ACID; PEROXYNITRITE; DESIGN;

D O I：

10.1016/j.saa.2023.122830

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The level of HClO/ClO- in mitochondria is essential to keep the normal function of mitochondria. Therefore, it is meaningful to accurately and quickly monitor ClO- in mitochondria. In this work, a new triphenylamine-based fluorescence probe PDTPA was designed and synthesized, in which pyridinium salt and dicyano-vinyl group were introduced as mitochondria targeting site and reaction site for ClO-. The probe showed high sensitivity and fast fluorescence response (<10 s) in the detection of ClO-. Moreover, the probe PDTPA had good linearity in a wide concentration range of ClO- and its detection limit was calculated as 10.5 & mu;M. Confocal fluorescence images demonstrated that the probe could target mitochondria and track the fluctuations of endogenous/ exogenous ClO- levels in the mitochondria of living cells.

引用

页数：8

共 58 条

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