Genome-Wide Identification and Expression Analysis of the MADS Gene Family in Tulips (Tulipa gesneriana)

被引:3
作者
Lu, Jiaojiao [1 ,2 ]
Qu, Lianwei [1 ]
Xing, Guimei [1 ]
Liu, Zhenlei [1 ]
Lu, Xiaochun [1 ]
Han, Xiaori [2 ]
机构
[1] Liaoning Acad Agr Sci, Shenyang 110161, Peoples R China
[2] Shenyang Agr Univ, Coll Land & Environm, Shenyang 110866, Peoples R China
关键词
MADS; gene expression; cold response; phylogeny; tulip; DEFICIENS-LIKE GENES; BOX GENES; SEPALOID TEPALS; DOMAIN PROTEINS; ABC-MODEL; EVOLUTION; SOFTWARE; SVP;
D O I
10.3390/genes14101974
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To investigate the cold response mechanism and low temperature regulation of flowering in tulips, this study identified 32 MADS-box transcription factor family members in tulips based on full-length transcriptome sequencing, named TgMADS1-TgMADS32. Phylogenetic analysis revealed that these genes can be divided into two classes: type I and type II. Structural analysis showed that TgMADS genes from different subfamilies have a similar distribution of conserved motifs. Quantitative real-time PCR results demonstrated that some TgMADS genes (e.g., TgMADS3, TgMADS15, TgMADS16, and TgMADS19) were significantly upregulated in buds and stems under cold conditions, implying their potential involvement in the cold response of tulips. In summary, this study systematically identified MADS family members in tulips and elucidated their evolutionary relationships, gene structures, and cold-responsive expression patterns, laying the foundation for further elucidating the roles of these transcription factors in flowering and the cold adaptability of tulips.
引用
收藏
页数:19
相关论文
共 67 条
[1]  
[Anonymous], 2005, BMC BIOINFORMATICS, V6
[2]  
[Anonymous], 2000, BIOTECH SOFTW INTERN, DOI DOI 10.1089/152791600459894
[3]   SignalP 5.0 improves signal peptide predictions using deep neural networks [J].
Armenteros, Jose Juan Almagro ;
Tsirigos, Konstantinos D. ;
Sonderby, Casper Kaae ;
Petersen, Thomas Nordahl ;
Winther, Ole ;
Brunak, Soren ;
von Heijne, Gunnar ;
Nielsen, Henrik .
NATURE BIOTECHNOLOGY, 2019, 37 (04) :420-+
[4]   MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress [J].
Arora, Rita ;
Agarwal, Pinky ;
Ray, Swatismita ;
Singh, Ashok Kumar ;
Singh, Vijay Pal ;
Tyagi, Akhilesh K. ;
Kapoor, Sanjay .
BMC GENOMICS, 2007, 8 (1)
[5]   MEME: discovering and analyzing DNA and protein sequence motifs [J].
Bailey, Timothy L. ;
Williams, Nadya ;
Misleh, Chris ;
Li, Wilfred W. .
NUCLEIC ACIDS RESEARCH, 2006, 34 :W369-W373
[6]   The major clades of MADS-box genes and their role in the development and evolution of flowering plants [J].
Becker, A ;
Theissen, G .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2003, 29 (03) :464-489
[7]   MG2C: a user-friendly online tool for drawing genetic maps [J].
Chao, Jiangtao ;
Li, Zhiyuan ;
Sun, Yuhe ;
Aluko, Oluwaseun Olayemi ;
Wu, Xinru ;
Wang, Qian ;
Liu, Guanshan .
MOLECULAR HORTICULTURE, 2021, 1 (01)
[8]   THE WAR OF THE WHORLS - GENETIC INTERACTIONS CONTROLLING FLOWER DEVELOPMENT [J].
COEN, ES ;
MEYEROWITZ, EM .
NATURE, 1991, 353 (6339) :31-37
[9]   Regulation of the floral repressor gene FLC: the complexity of transcription in a chromatin context [J].
Crevillen, Pedro ;
Dean, Caroline .
CURRENT OPINION IN PLANT BIOLOGY, 2011, 14 (01) :38-44
[10]  
Eddy Sean R, 2009, Genome Inform, V23, P205