Antagonistic effects of volatile organic compounds of Saccharomyces cerevisiae NJ-1 on the growth and toxicity of Aspergillus flavus

被引：12

作者：

Yang, Ting ^{[1
]}

Wang, Chengzhong ^{[1
]}

Li, Chenjie ^{[1
]}

Sun, Rui ^{[1
]}

Yang, Mingguan ^{[1
,2
]}

机构：

[1] Qilu Univ Technol, Shandong Acad Sci, Coll Food Sci & Engn, Jinan 250353, Peoples R China

[2] Qilu Univ Technol, Shandong Acad Sci, 3501 Daxue Rd, Jinan, Peoples R China

来源：

BIOLOGICAL CONTROL | 2023年 / 177卷

关键词：

Aspergillus flavus; Biological control; Saccharomyces cerevisiae NJ-1; Volatile organic compounds; STREPTOMYCES-ALBOFLAVUS TD-1; ANTIBACTERIAL ACTIVITY; ESCHERICHIA-COLI; ESSENTIAL OIL; MECHANISM; EXPRESSION;

D O I：

10.1016/j.biocontrol.2022.105093

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aspergillus flavus is capable of producing highly toxic aflatoxins that can infest grains, cereals and nuts, causing serious economic losses and food safety concerns. Therefore, it is especially necessary to study safe and efficient biological control methods. In this paper, an edible strain of Saccharomyces cerevisiae NJ-1 was isolated from pomace of Ficus carica, the inhibitory effects of S. cerevisiae NJ-1 volatile organic compounds (VOCs) on the growth and aflatoxin production of A. flavus were studied, and the mechanisms of inhibitory effects were initially investigated. The results revealed that the inhibitory effects of S. cerevisiae NJ-1 VOCs on the growth, sporulation, and conidial germination of A. flavus varied significantly with the preculture time. Scanning electron microscopy (SEM) images showed that the morphology of the mycelium of A. flavus treated with VOCs was distorted. Meanwhile, the absorbance at 260 nm and 280 nm had a significant increasing trend, which indicated that the cell membrane was one of the action targets of S. cerevisiae NJ-1 VOCs. A total of nine compounds were identified from S. cerevisiae NJ-1 VOCs by headspace solid-phase microextraction gas chromatography (GC/MS). Among them, 3-methyl-1-butanol showed a significant inhibitory effect on A. flavus and may play an important role in the inhibitory effect of NJ-1 VOCs. The study showed that S. cerevisiae NJ-1 VOCs have great potential to be developed as biopesticides for the control of A. flavus.

引用

页数：7

共 32 条

[11] Study on inhibitory activity and mechanism of chitosan oligosaccharides on Aspergillus Flavus and Aspergillus Fumigatus [J].

Ke, Yuan ;

Ding, Beibei ;

Zhang, Miaomiao ;

Dong, Tongjun ;

Fu, Yang ;

Lv, Qingyun ;

Ding, Wenping ;

Wang, Xuedong .

CARBOHYDRATE POLYMERS, 2022, 275

[12]

Li Q., 2021, GOST, V4, P131, DOI [10.1016/j.gaost.2021.07.002, DOI 10.1016/J.GAOST.2021.07.002]

[13] Biofumigation on Post-Harvest Diseases of Fruits Using a New Volatile-Producing Fungus of Ceratocystis fimbriata [J].

Li, Qian ;

Wu, Lei ;

Hao, Jianjun ;

Luo, Laixin ;

Cao, Yongsong ;

Li, Jianqiang .

PLOS ONE, 2015, 10 (07)

[14] Biological Control Products for Aflatoxin Prevention in Italy: Commercial Field Evaluation of Atoxigenic Aspergillus flavus Active Ingredients [J].

Mauro, Antonio ;

Garcia-Cela, Esther ;

Pietri, Amedeo ;

Cotty, Peter J. ;

Battilani, Paola .

TOXINS, 2018, 10 (01)

[15] The potential role of fungal volatile organic compounds in Aspergillus flavus biocontrol efficacy [J].

Moore, Geromy G. ;

Lebar, Matthew D. ;

Carter-Wientjes, Carol H. ;

Gilbert, Matthew K. .

BIOLOGICAL CONTROL, 2021, 160

[16] Volatile organic compounds from Wickerhamomyces anomalus, Metschnikowia pulcherrima and Saccharomyces cerevisiae inhibit growth of decay causing fungi and control postharvest diseases of strawberries [J].

Oro, Lucia ;

Feliziani, Erica ;

Ciani, Maurizio ;

Romanazzi, Gianfranco ;

Comitini, Francesca .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2018, 265 :18-22

[17] Crop molds and mycotoxins: Alternative management using biocontrol [J].

Phuong-Anh Nguyen ;

Strub, Caroline ;

Fontana, Angelique ;

Schorr-Galindo, Sabine .

BIOLOGICAL CONTROL, 2017, 104 :10-27

[18] Upland rice vinegar vapor inhibits spore germination, hyphal growth and aflatoxin formation in Aspergillus flavus on maize grains [J].

Pornpukdeewattana, Soisuda ;

Kerdpiboon, Soraya ;

Jindaprasert, Aphacha ;

Pandee, Patcharaporn ;

Teerarak, Montinee ;

Krusong, Warawut .

FOOD CONTROL, 2017, 71 :88-93

[19]

Reddy B.N., 2017, VOLATILES FOOD SECUR, DOI [10.1007/978-981-10-5553-9_14, DOI 10.1007/978-981-10-5553-9_14]

[20] Invasive Aspergillosis by Aspergillus flavus: Epidemiology, Diagnosis, Antifungal Resistance, and Management [J].

Rudramurthy, Shivaprakash M. ;

Paul, Raees A. ;

Chakrabarti, Arunaloke ;

Mouton, Johan W. ;

Meis, Jacques F. .

JOURNAL OF FUNGI, 2019, 5 (03)

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