Identification and Characterization of Antibiotic-Resistant, Gram-Negative Bacteria Isolated from Korean Fresh Produce and Agricultural Environment

被引:5
作者
Jeong, Sunyoung [1 ,2 ]
Kim, Ile [1 ,3 ]
Kim, Bo-Eun [4 ]
Jeong, Myeong-In [4 ]
Oh, Kwang-Kyo [4 ]
Cho, Gyu-Sung [1 ]
Franz, Charles M. A. P. [1 ]
机构
[1] Fed Res Inst Nutr & Food, Max Rubner Inst, Dept Microbiol & Biotechnol, Hermann Weigmann Str 1, D-24103 Kiel, Germany
[2] Korea Univ, Coll Life Sci & Biotechnol, Anam Ro, Seoul 02841, South Korea
[3] Handong Global Univ, Dept Life Sci, 558 Handong Ro, Pohang 37554, South Korea
[4] Rural Dev Adm, Natl Inst Agr Sci, Microbial Safety Div, 166 Nongsaengmyeong Ro,Iseo Myeon, Wonju 55365, South Korea
关键词
antibiotic resistance; lettuce; fresh produce; whole genome sequencing; ESCHERICHIA-COLI; ANTIMICROBIAL RESISTANCE; VEGETABLES; PREVALENCE; ALGORITHM; PATHOGENS; PLASMIDS;
D O I
10.3390/microorganisms11051241
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The consumption of fresh produce and fruits has increased over the last few years as a result of increasing consumer awareness of healthy lifestyles. Several studies have shown that fresh produces and fruits could be potential sources of human pathogens and antibiotic-resistant bacteria. In this study, 248 strains were isolated from lettuce and surrounding soil samples, and 202 single isolates selected by the random amplified polymorphic DNA (RAPD) fingerprinting method were further characterized. From 202 strains, 184 (91.2%) could be identified based on 16S rRNA gene sequencing, while 18 isolates (8.9%) could not be unequivocally identified. A total of 133 (69.3%) and 105 (54.7%) strains showed a resistance phenotype to ampicillin and cefoxitin, respectively, while resistance to gentamicin, tobramycin, ciprofloxacin, and tetracycline occurred only at low incidences. A closer investigation of selected strains by whole genome sequencing showed that seven of the fifteen sequenced strains did not possess any genes related to acquired antibiotic resistance. In addition, only one strain possessed potentially transferable antibiotic resistance genes together with plasmid-related sequences. Therefore, this study indicates that there is a low possibility of transferring antibiotic resistance by potential pathogenic enterobacteria via fresh produce in Korea. However, with regards to public health and consumer safety, fresh produce should nevertheless be continuously monitored to detect the occurrence of foodborne pathogens and to hinder the transfer of antibiotic resistance genes potentially present in these bacteria.
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页数:16
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