Engineered GO-Silk Fibroin-Based Hydrogel for the Promotion of Collagen Synthesis in Full-Thickness Skin Defect

被引：1
作者：
Syromiatnikova, Valeriia [1 ]
Gupta, Sharda [2 ]
Zhuravleva, Margarita [1 ]
Masgutova, Galina [1 ]
Zakirova, Elena [1 ]
Aimaletdinov, Alexander [1 ]
Rizvanov, Albert [1 ]
Salafutdinov, Ilnur [1 ,3 ]
Naumenko, Ekaterina [1 ]
Bit, Arindam [2 ]
机构：
[1] Kazan Fed Univ, Inst Fundamental Med & Biol, Kazan 420008, Russia
[2] Natl Inst Technol, Dept Biomed Engn, Raipur 492001, India
[3] Kazan State Med Univ, Dept Med Biol & Genet, Kazan 420012, Russia
来源：
JOURNAL OF COMPOSITES SCIENCE | 2023年 / 7卷 / 05期
关键词：
hydrogel; chitosan; silk fibroin; graphene oxide; skin wounds; GRAPHENE; UPDATE;
D O I：
10.3390/jcs7050186
中图分类号：
TB33 [复合材料];
学科分类号：
摘要：
In order to improve the regeneration of full-layer skin defects, hydrogels were developed based on the combination of chitosan (Cs), Daba silk fibroin (DSF), and graphene oxide (GO): CS, DSF/Cs and DSF/Cs/GO. The biocompatibility of hydrogels with human dermis fibroblasts in vitro was evaluated using the MTS assay. To assess the regenerative potential of hydrogels, a model of a full-layer skin defect was reconstructed on the back of rats and closed the wound surface with CS, DSF/Cs and DSF/Cs/GO hydrogels. The morphological and morphometric characteristics of regenerate tissues were obtained by staining with hematoxylin-eosin, Heidengain azocarmine, and immunohistochemistry on days 7 and 14 of the experiment. It has been shown that the use of DSF/Cs and DSF/Cs/GO promotes enhanced healing and epithelization of a full-layer skin wound. The addition of GO to the hydrogel increased the synthetic activity of fibroblasts and improved the characteristics of the produced collagen fibers.
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页数：14
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