Engineering a Point-of-Care Paper-Microfluidic Electrochemical Device Applied to the Multiplexed Quantitative Detection of Biomarkers in Sputum

被引：19

作者：

Gutierrez-Capitan, Manuel ^{[1
]}

Sanchis, Ana ^{[2
,3
]}

Carvalho, Estela O. ^{[4
,5
]}

Baldi, Antonio ^{[1
]}

Vilaplana, Lluisa ^{[2
,3
]}

Cardoso, Vanessa F. ^{[4
,5
,6
]}

Calleja, Alvaro ^{[1
]}

Wei, Mingxing ^{[7
]}

de la Rica, Roberto ^{[8
,9
]}

Hoyo, Javier ^{[10
]}

Bassegoda, Arnau ^{[10
]}

Tzanov, Tzanko ^{[10
]}

Marco, Maria-Pilar ^{[2
,3
]}

Lanceros-Mendez, Senentxu ^{[4
,5
,11
]}

Fernandez-Sanchez, Cesar ^{[1
,3
,12
]}

机构：

[1] CSIC, IMB, CNM, Bellaterra 08193, Spain

[2] CSIC, Inst Adv Chem Catalonia IQAC, Nanobiotechnol Diagnost Nb4D, Barcelona 08034, Spain

[3] Ctr Invest Biomed Red Bioingn Biomat, Madrid 28029, Spain

[4] Univ Minho & Porto CF UM UP, Ctr Phys, P-4710057 Braga, Portugal

[5] LaPMET, P-4710057 Braga, Portugal

[6] CMEMS UMinho, P-4800058 Guimaraes, Portugal

[7] SAS, Cellvax, F-94800 Villejuif, France

[8] Hlth Res Inst Balear Isl IdISBa, Multidisciplinary Sepsis Grp, Palma De Mallorca 07120, Spain

[9] Ctr Invest Biomed Red Enfermedades Infecciosas, Madrid 28029, Spain

[10] Univ Politecn Cataluna, Dept Engn Quim, Grup Biotecnol Mol & Ind, Terrassa 08222, Spain

[11] Univ Basque Country, Basque Ctr Mat & Applicat BCMat, Leioa 48940, Spain

[12] IKERBASQUE, Bilbao 48009, Spain

来源：

ACS SENSORS | 2023年 / 8卷 / 08期

关键词：

point-of-care rapid tests; chronic obstructive pulmonarydisease; electrochemical biosensing; paper microfluidics; magnetic nanoparticle-based immunoassay; multiplexeddetection; OBSTRUCTIVE PULMONARY-DISEASE; ASSAY; BIOSENSORS;

D O I：

10.1021/acssensors.3c00523

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Health initiatives worldwide demand affordable point-of-caredevicesto aid in the reduction of morbidity and mortality rates of high-incidenceinfectious and noncommunicable diseases. However, the production ofrobust and reliable easy-to-use diagnostic platforms showing the abilityto quantitatively measure several biomarkers in physiological fluidsand that could in turn be decentralized to reach any relevant environmentremains a challenge. Here, we show the particular combination of paper-microfluidictechnology, electrochemical transduction, and magnetic nanoparticle-basedimmunoassay approaches to produce a unique, compact, and easily deployablemultiplex device to simultaneously measure interleukin-8, tumor necrosisfactor-& alpha;, and myeloperoxidase biomarkers in sputum, developedwith the aim of facilitating the timely detection of acute exacerbationsof chronic obstructive pulmonary disease. The device incorporatesan on-chip electrochemical cell array and a multichannel paper component,engineered to be easily aligned into a polymeric cartridge and exchangedif necessary. Calibration curves at clinically relevant biomarkerconcentration ranges are produced in buffer and artificial sputum.The analysis of sputum samples of healthy individuals and acutelyexacerbated patients produces statistically significant biomarkerconcentration differences between the two studied groups. The devicecan be mass-produced at a low cost, being an easily adaptable platformfor measuring other disease-related target biomarkers.

引用

页码：3032 / 3042

页数：11

共 54 条

[1] Persistent Systemic Inflammation is Associated with Poor Clinical Outcomes in COPD: A Novel Phenotype [J].