Apolipoprotein-E genotyping in formalin-fixed and paraffin-embedded post-mortem brain tissue

被引：1

作者：

Minshull, James ^{[1
,2
,4
]}

Davidson, Yvonne ^{[1
,2
]}

Roncaroli, Federico ^{[1
,2
,3
]}

Robinson, Andrew C. ^{[1
,2
]}

机构：

[1] Univ Manchester, Fac Biol Med & Hlth, Sch Biol Sci, Div Neurosci, Manchester, England

[2] Manchester Acad Hlth Sci Ctr MAHSC, Geoffrey Jefferson Brain Res Ctr, Manchester, England

[3] Salford Royal Hosp, Northern Care Alliance, Salford, England

[4] Univ Manchester, Salford Royal Hosp, Clin Sci Bldg, Salford M6 8HD, England

来源：

BRAIN PATHOLOGY | 2024年 / 34卷 / 05期

关键词：

Alzheimer's disease; APOE; genotyping techniques; neurodegenerative diseases; PCR; ASSOCIATION; AGE; DNA;

D O I：

10.1111/bpa.13243

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Formalin-fixed paraffin-embedded (FFPE) brain tissue held in tissue banks constitutes a valuable research resource, especially when associated with clinical annotations and longitudinal psychometric testing. Apolipoprotein-E (APOE) genotyping is important to fully characterise this resource, however older FFPE tissue may not be suitable for genotyping. We performed polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays on DNA extracted from post-mortem FFPE brain tissue ranging from 2-19 years old. A maximum of three years in paraffin was determined for robust APOE genotyping of FFPE tissue using PCR-RFLP which may suggest prolonged storage of fixed tissue as FFPE blocks may have deleterious effects on DNA.

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