The pivotal role of neuronal nitric oxide synthase in the release of 6-nitrodopamine from mouse isolated vas deferens

被引：4

作者：

Britto-Junior, Jose ^{[1
,7
]}

Silva, Samuel Goulart Nacario ^{[1
]}

Lima, Antonio Tiago ^{[1
]}

Fuguhara, Vivian ^{[1
]}

Andrade, Larissa Bueno ^{[1
]}

Mendes, Gustavo Duarte ^{[2
,3
]}

Peterson, Larryn W. ^{[4
]}

Chiavegatto, Silvana ^{[5
,6
]}

Antunes, Edson ^{[1
]}

De Nucci, Gilberto ^{[1
,2
,3
,4
]}

机构：

[1] Univ Estadual Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, Brazil

[2] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, Campinas, SP, Brazil

[3] Univ Metropolitana Santos, Fac Med, Dept Pharmacol, Santos, SP, Brazil

[4] Rhodes Coll, Dept Chem, Memphis, TN 38112 USA

[5] Univ Sao Paulo ICB USP, Inst Biomed Sci ICB, Dept Pharmacol, Sao Paulo, Brazil

[6] Univ Sao Paulo Med Sch FMUSP, Inst Psychiat IPq, Dept Psychiat, Sao Paulo, Brazil

[7] Univ Campinas Unicamp, Fac Med Sci, Dept Pharmacol, Rua Tessalia Vieira Camargo,126 Cidade Univ, BR-13083887 Campinas, SP, Brazil

来源：

NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2024年 / 143卷

基金：

巴西圣保罗研究基金会;

关键词：

Liquid chromatography; Tandem mass spectrometry; Dopamine; Noradrenaline; Adrenaline; Catecholamine; NERVE; 6-HYDROXYDOPAMINE; OXIDATION; DOPAMINE;

D O I：

10.1016/j.niox.2023.12.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

6-Nitrodopamine (6-ND) is released from rat and human vas deferens and is considered a major mediator of both tissues contractility. The contractions induced by 6-ND are selectively blocked by both tricyclic antidepressants and alpha 1-adrenoceptor antagonists. Endothelial nitric oxide synthase (eNOS) is the major isoform responsible for 6ND release in mouse isolated heart, however the origin of 6-ND in the vas deferens is unknown. Here it was investigated by LC-MS/MS the basal release of 6-ND from isolated vas deferens obtained from control, eNOS-/-, nNOS-/-, and iNOS- /- mice. In addition, it was evaluated in vitro vas deferens contractility following electric field stimulation (EFS).Basal release of 6-ND was significantly reduced in nNOS-/- mice compared to control mice, but not decreased when the vas deferens were obtained from either eNOS-/- or iNOS-/- mice. Pre-incubation of the vas deferens with tetrodotoxin (1 mu M) significantly reduced the basal release of 6-ND from control, eNOS- /-, and iNOS-/mice but had no effect on the basal release of 6-ND from nNOS-/- mice. EFS-induced frequency-dependent contractions of the vas deferens, which were significantly reduced when the tissues obtained from control, eNOS-/- and iNOS- /- mice, were pre-incubated with L-NAME, but unaltered when the vas deferens was obtained from nNOS-/- mice. In addition, the EFS-induced contractions were significantly smaller when the vas deferens were obtained from nNOS-/- mice.The results clearly demonstrate that nNOS is the main NO isoform responsible for 6-ND release in mouse vas deferens and reinforces the concept of 6-ND as a major modulator of vas deferens contractility.

引用

页码：1 / 8

页数：8

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