Polymer-Protein Assemblies with Tunable Vesicular and Hierarchical Nanostructures

The construction of variable structured multi-protein nano-assemblies is of great interest for the development of protein-based therapeutic systems. This study showcases the synthesis of polymer-protein assemblies with tunable structure like single- and multi-layer polymer-crosslinked protein vesicles, Janus protein vesicles and other hierarchical-structured assemblies by utilizing a dynamic template-assistant intermittent-assembly approach. The generalization of the methodology helps the protein assemblies to gain notable functional complexity. And we demonstrate compelling evidence highlighting the substantial impact of the topological morphology of protein nanoaggregates on their cellular uptake capacity. This study describes the synthesis of polymer-crosslinked protein assemblies with tunable microstructures, including single- and multi-layer protein vesicles, Janus protein vesicles and doughnut-shaped hierarchical-structured protein aggregates. The as-prepared protein aggregates display morphology-dependent cell uptake.image