ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway

被引:3
作者
Zhang, Guanli [1 ]
Feng, Yan [2 ]
Wang, Min [2 ]
Liu, Xin [2 ]
机构
[1] Yantaishan Hosp, Dept Obstet, Yantai, Shandong, Peoples R China
[2] Liaocheng Dongchangfu Maternal & Child Hlth Hosp, Dept Obstet, 129 Zhenxing Rd, Liaocheng 252000, Shandong, Peoples R China
关键词
invasion; migration; mTOR pathway; pre-eclampsia; proliferation; PI3K/AKT/MTOR PATHWAY; PLACENTAL CYTOTROPHOBLASTS; ENDOTHELIAL-CELLS; PREECLAMPSIA; PATHOGENESIS; PROTEIN; ACTIVATION; PREGNANCY; REQUIRES; GTPASES;
D O I
10.1002/jbt.23237
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pre-eclampsia (PE) is a major cause of hypertension in maternal and fetal. Atlastin-1 (ATL1), one regulator of endoplasmic reticulum (ER) morphology, participates in tubular ER formation and protein synthesis. The objective of this study is to investigate the role and molecular mechanism of ATL1 in PE. GEO databases showed that ATL1 was upregulated in PE patients. Our data also found that ATL1 was highly expressed in PE placental tissues. The cell viability, proliferation, migration, and invasion of HTR-8/SVneo cells increased/decreased after the downregulation/upregulation of ATL1. The mTOR pathway is the downstream pathway of ATL1. The levels of p-p70S6K and p-mTOR were increased/decreased after the downregulation/upregulation of ATL1. Moreover, rapamycin, an inhibitor of mTOR pathway, reversed the promotive effect of siATL1 on proliferation, migration, and invasion in HTR-8/SVneo cells. In conclusion, ATL1 inhibits the proliferation and invasion of trophoblast cells via the inhibition of the mTOR signaling pathway in HTR-8/SVneo cells.
引用
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页数:9
相关论文
共 61 条
[1]   The metastasis-associated Mts1(S100A4) protein could act as an angiogenic factor [J].
Ambartsumian, N ;
Klingelhöfer, J ;
Grigorian, M ;
Christensen, C ;
Kriajevska, M ;
Tulchinsky, E ;
Georgiev, G ;
Berezin, V ;
Bock, E ;
Rygaard, J ;
Cao, RH ;
Cao, YH ;
Lukanidin, E .
ONCOGENE, 2001, 20 (34) :4685-4695
[2]   Pre-eclampsia rates in the United States, 1980-2010: age-period-cohort analysis [J].
Ananth, Cande V. ;
Keyes, Katherine M. ;
Wapner, Ronald J. .
BMJ-BRITISH MEDICAL JOURNAL, 2013, 347
[3]   Oxidative Stress in Preeclampsia and Placental Diseases [J].
Aouache, Rajaa ;
Biquard, Louise ;
Vaiman, Daniel ;
Miralles, Francisco .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (05)
[4]   Intrauterine growth restriction and placental angiogenesis [J].
Barut, Figen ;
Barut, Aykut ;
Gun, Banu Dogan ;
Kandemir, Nilufer Onak ;
Harma, Mehmet Ibrahim ;
Harma, Muge ;
Aktunc, Erol ;
Ozdamar, Sukru Oguz .
DIAGNOSTIC PATHOLOGY, 2010, 5
[5]   CDK9 and mTOR: trading places [J].
Borden, Katherine L. B. .
BLOOD, 2019, 133 (11) :1167-1168
[6]   Oxygen and placental development during the first trimester: Implications for the pathophysiology of pre-eclampsia [J].
Caniggia, I ;
Winter, J ;
Lye, SJ ;
Post, M .
PLACENTA, 2000, 21 :S25-S30
[7]   Toll-like receptor-mediated induction of type I interferon in plasmacytoid dendritic cells requires the rapamycin-sensitive PI(3) K-mTOR-p70S6K pathway [J].
Cao, Weiping ;
Manicassamy, Santhakumar ;
Tang, Hua ;
Kasturi, Sudhir Pai ;
Pirani, Ali ;
Murthy, Niren ;
Pulendran, Bali .
NATURE IMMUNOLOGY, 2008, 9 (10) :1157-1164
[8]   Cryo-EM structure of human mTOR complex 2 [J].
Chen, Xizi ;
Liu, Mengjie ;
Tian, Yuan ;
Li, Jiabei ;
Qi, Yilun ;
Zhao, Dan ;
Wu, Zihan ;
Huang, Min ;
Wong, Catherine C. L. ;
Wang, Hong-Wei ;
Wang, Jiawei ;
Yang, Huirong ;
Xu, Yanhui .
CELL RESEARCH, 2018, 28 (05) :518-528
[9]   Found in translation of mTOR signaling [J].
Clohessy, John G. ;
Reschke, Markus ;
Pandolfi, Pier Paolo .
CELL RESEARCH, 2012, 22 (09) :1315-1318
[10]  
Cunningham M.F., 2010, AM J PHYSIOL-GASTR L, V299, P20