Electrochemiluminescence Amplification in Bead-Based Assays Induced by a Freely Diffusing Iridium(III) Complex

被引:32
作者
Kerr, Emily [1 ]
Knezevic, Sara [2 ]
Francis, Paul S. [3 ]
Hogan, Conor F. [4 ]
Valenti, Giovanni [5 ]
Paolucci, Francesco [5 ]
Kanoufi, Frederic [6 ]
Sojic, Neso [2 ]
机构
[1] Deakin Univ, Inst Frontier Mat, Geelong, Vic 3220, Australia
[2] Univ Bordeaux, CNRS, UMR 5255, Bordeaux INP,Inst Sci Mol, F-33607 Pessac, France
[3] Deakin Univ, Sch Life & Environm Sci, Geelong, Vic 3220, Australia
[4] La Trobe Univ, La Trobe Inst Mol Sci, Biomed & Environm Sensor Technol Ctr, Dept Biochem & Chem, Melbourne, Vic 3086, Australia
[5] Univ Bologna, Dept Chem Giacomo Ciamician, I-40126 Bologna, Italy
[6] Univ Paris Cite, CNRS, ITODYS, F-75013 Paris, France
基金
英国医学研究理事会;
关键词
electrochemiluminescence; imaging; bead-based assays; heterogeneous assays; electrochemistry; TRI-N-PROPYLAMINE; ELECTROGENERATED CHEMILUMINESCENCE; ENHANCED ELECTROCHEMILUMINESCENCE; OXIDATION; QUANTIFICATION; RUTHENIUM(II); IMMUNOASSAYS; POTENTIALS; STRATEGY; CATION;
D O I
10.1021/acssensors.2c02697
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Heterogeneous electrochemiluminescence (ECL) assays employing tri-n-propylamine as a co-reactant and a tris(2,2 '-bipyridine)ruthenium(II) ([Ru(bpy)3]2+) derivative as an emissive label are integral to the majority of academic and commercial applications of ECL sensing. This model system is an active research area and constitutes the basis of successfully commercialized bead-based ECL immunoassays. Herein, we propose a novel approach to the enhancement of such conven-tional ECL assays via the incorporation of a second metal coordination complex, [Ir(sppy)3]3- (where sppy = 5 '-sulfo-2-phenylpyridinato-C2,N), to the experimental system. By employing ECL microscopy, we are able to map the spatial distribution of ECL emission at the surface of the bead, from [Ru(bpy)3]2+ labels, and solution-phase emission, from [Ir(sppy)3]3-. The developed [Ir(sppy)3]3--mediated enhancement approach elicited a significant improvement (70.9-fold at 0.9 V and 2.9-fold at 1.2 V vs Ag/AgCl) of the ECL signal from [Ru(bpy)3]2+ labels immobilized on the surface of a polystyrene bead. This dramatic enhancement in ECL signal, particularly at low oxidation potentials, has important implications for the improvement of existing heterogeneous ECL assays and ECL-based microscopy, by amplifying the signal, new detection and both electrode surface and deleterious side reactions.
引用
收藏
页码:933 / 939
页数:7
相关论文
共 42 条
[1]   Absolute Electrochemiluminescence Efficiency Quantification Strategy Exemplified with Ru(bpy)32+ in the Annihilation Pathway [J].
Adsetts, Jonathan R. ;
Chu, Kenneth ;
Hesari, Mahdi ;
Ma, Jing ;
Ding, Zhifeng .
ANALYTICAL CHEMISTRY, 2021, 93 (33) :11626-11633
[2]   Physical Strategy to Determine Absolute Electrochemiluminescence Quantum Efficiencies of Coreactant Systems Using a Photon-Counting Photomultiplier Device [J].
Chu, Kenneth ;
Adsetts, Jonathan R. ;
Ma, Jing ;
Zhang, Congyang ;
Hesari, Mahdi ;
Yang, Liuqing ;
Ding, Zhifeng .
JOURNAL OF PHYSICAL CHEMISTRY C, 2021, 125 (40) :22274-22282
[3]   Theory and Simulation for Optimising Electrogenerated Chemiluminescence from Tris(2,2′-bipyridine)-ruthenium(II)-Doped Silica Nanoparticles and Tripropylamine [J].
Daviddi, Enrico ;
Oleinick, Alexander ;
Svir, Irina ;
Valenti, Giovanni ;
Paolucci, Francesco ;
Amatore, Christian .
CHEMELECTROCHEM, 2017, 4 (07) :1719-1730
[4]   Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level [J].
Deiss, Frederique ;
LaFratta, Christopher N. ;
Symer, Matthew ;
Blicharz, Timothy M. ;
Sojic, Neso ;
Walt, David R. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (17) :6088-+
[5]   Reactivity mapping of luminescence in space: Insights into heterogeneous electrochemiluminescence bioassays [J].
Dutta, Priyanka ;
Han, Dongni ;
Goudeau, Bertrand ;
Jiang, Dechen ;
Fang, Danjun ;
Sojic, Neso .
BIOSENSORS & BIOELECTRONICS, 2020, 165
[6]  
Faatz E, 2020, RSC DETECT SCI SER, V15, P443
[7]   Photophysics and Electrochemiluminescence of Bright Cyclometalated Ir(III) Complexes in Aqueous Solutions [J].
Fernandez-Hernandez, Jesus M. ;
Longhi, Elena ;
Cysewski, Robert ;
Polo, Federico ;
Josel, Hans-Peter ;
De Cola, Luisa .
ANALYTICAL CHEMISTRY, 2016, 88 (08) :4174-4178
[8]   Spatially resolved electrochemiluminescence through a chemical lens [J].
Fiorani, Andrea ;
Han, Dongni ;
Jiang, Dechen ;
Fang, Danjun ;
Paolucci, Francesco ;
Sojic, Neso ;
Valenti, Giovanni .
CHEMICAL SCIENCE, 2020, 11 (38) :10496-10500
[9]   Quantification of electrogenerated chemiluminescence from tris(bipyridine)ruthenium(ii) and hydroxyl ions [J].
Fiorani, Andrea ;
Valenti, Giovanni ;
Irkham ;
Paolucci, Francesco ;
Einaga, Yasuaki .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2020, 22 (27) :15413-15417
[10]   Microtube Electrodes for Imaging the Electrochemiluminescence Layer and Deciphering the Reaction Mechanism [J].
Guo, Weiliang ;
Zhou, Ping ;
Sun, Lei ;
Ding, Hao ;
Su, Bin .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 60 (04) :2089-2093