Effects of YAP1 on proliferation and differentiation of Hu sheep skeletal muscle satellite cells in vitro

被引：4

作者：

He, Mingliang ^{[1
,2
]}

Zhang, Weibo ^{[1
,2
]}

Wang, Shan ^{[1
,2
]}

Ge, Ling ^{[1
,2
]}

Cao, Xiukai ^{[2
,3
]}

Wang, Shanhe ^{[1
,2
]}

Yuan, Zehu ^{[2
,3
]}

Lv, Xiaoyang ^{[2
,3
]}

Getachew, Tesfaye ^{[2
,4
]}

Mwacharo, Joram M. ^{[2
,4
]}

Haile, Aynalem ^{[2
,4
]}

Sun, Wei ^{[1
,2
,3
]}

机构：

[1] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China

[2] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Jiangsu, Peoples R China

[3] Int Joint Res Lab Univ Jiangsu Prov China Domest, Nanjing, Peoples R China

[4] Int Ctr Agr Res Dry Areas, Addis Ababa, Ethiopia

来源：

ANIMAL BIOTECHNOLOGY | 2023年 / 34卷 / 07期

基金：

中国国家自然科学基金;

关键词：

YAP1; proliferation; differentiation; satellite cell; Hu sheep; PATHWAY;

D O I：

10.1080/10495398.2022.2112688

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study aimed to understand the expression level of YAP1 in the skeletal muscle of Hu sheep and to reveal the regulatory mechanism of YAP1 on Hu sheep skeletal muscle satellite cells (SMSCs). Previous research by our group has found that YAP1 may affect the growth and development of Hu sheep skeletal muscle. In the present study, we found the expression of YAP1 in the skeletal muscle is higher than in other tissues of Hu sheep. Then, we detected the effect of YAP1 on proliferation and differentiation in Hu sheep SMSCs. According to the results of qPCR, CCK-8, EDU, and Western blot, compared to the group of negative control, overexpression of YAP1 promoted the proliferation and inhibited the differentiation of SMSCs according to the results of qPCR, CCK-8, EDU, Western blot, while the interference of YAP1 was on the contrary. Overall, our study suggests that YAP1 is an important functional molecule in the growth and development of skeletal muscle by regulating the proliferation and differentiation of SMSCs. These findings are of great use for understanding the roles of YAP1 in the skeletal muscle of Hu sheep.

引用

页码：2691 / 2700

页数：10

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