UP-regulated levels of sHLA-G in women with a history of RPL in mid-gestation presumably to achieve ongoing pregnancy

被引：0

作者：

Jahan, Parveen ^{[1
]}

Bhuwalka, Rashmi ^{[1
]}

Begum, Mahmooda ^{[1
]}

Madduru, Dhatri ^{[2
]}

Ahmad, Arif ^{[1
]}

Bonu, Rajeshwari ^{[3
]}

机构：

[1] Maulana Azad Natl Urdu Univ Gachibowli, Sch Sci Zool, Hyderabad, Telangana, India

[2] Osmania Univ, Dept Biochem, Hyderabad, Telangana, India

[3] Niloufer Hosp, Gynaecol & Obstet Dept, Hyderabad, Telangana, India

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2023年 / 90卷 / 06期

关键词：

CD4+HLA-G+ tTreg; iRPL; ROC; sHLA-G; LEUKOCYTE ANTIGEN-G; HLA-G EXPRESSION; T-CELLS; G MOLECULE; CD4(+); SYSTEM; PREECLAMPSIA; TOLERANCE; PLASMA; BLOOD;

D O I：

10.1111/aji.13798

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ProblemRecurrent Pregnancy Loss (RPL) is a disorder characterized by two or more pregnancy losses within 20th week of gestation. Globally 1-5% of the couples are affected, 50% of these cases are with unknown etiology. HLA-G, an Immuno-modulatory molecule is a non-classical MHC-1 protein, expressed abundantly on extravillous trophoblastic cells, responsible for spiral artery remodeling, maintaining maternal immune tolerance and fetal growth by adjusting pro and anti-inflammatory milieu during different gestational phases.Method of studyIn the present case-control study CD4+HLA-G+ tTreg cells were enumerated by flow cytometry and estimation of the circulating levels of sHLA-G in the blood samples of 300 mid-gestation pregnant women with (iRPL) and without history of RPL (nRPL) by Enzyme-linked Immunosorbent assay was done. The cases included 92 primary and 58 secondary RPL casesResultsA significant reduction in number of tTregs and elevated levels of circulating sHLA-G in iRPL (.03, 200.9) versus nRPL (.09, 90.32) was observed. Further, the primary cases showed higher circulating sHLA-G and no difference in relation to CD4+HLA-G+ tTregs compared to the secondary cases. Receiver operating curve (ROC) characteristics of sHLA-G (AUC = .8) was superior to CD4+HLA-G+ (AUC = .7) for iRPL patients over nRPL group. Conclusions: Our results are suggestive of the over-expression of sHLA-G which may be caused due to its shedding from surface of trophoblast as a compensatory mechanism to save the on-going pregnancy. To realize the present outcome, studies are required on on-going pregnancy follow-up cases with favorable and unfavorable pregnancy outcome.

引用

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