Vitamin D delays intervertebral disc degeneration and improves bone quality in ovariectomized rats

被引:3
作者
Xu, Hao-Wei [1 ]
Fang, Xin-Yue [1 ]
Chen, Hao [1 ]
Zhang, Shu-Bao [1 ]
Yi, Yu-Yang [1 ]
Ge, Xiao-Yong [1 ]
Liu, Xiao-Wei [1 ]
Wang, Shan-Jin [1 ,2 ,3 ,4 ]
机构
[1] Tongji Univ, Shanghai East Hosp, Sch Med, Dept Spinal Surg, Shanghai, Peoples R China
[2] Jinggangshan Univ, Inst Spinal Dis, Jian, Jiangxi, Peoples R China
[3] JiAn Hosp, Shanghai East Hosp, Dept Orthoped, Jian, Jiangxi, Peoples R China
[4] 150 Jimo RD, Shanghai 200120, Peoples R China
关键词
antioxidant enzymes; endplate porosity; intervertebral disc degeneration; ovariectomy; vitamin D; OSTEOPOROSIS; WOMEN;
D O I
10.1002/jor.25778
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Known to be involved in bone-cartilage metabolism, Vitamin D (VD) may play a role in human's disc pathophysiology. Given that postmenopausal women are prone to suffer VD deficiency and intervertebral disc degeneration (IDD), this study is intended to investigate whether VD can delay IDD in ovariectomized rats by improving bone microstructure and antioxidant stress. Female Sprague-Dawley rats were randomly allocated into four groups: sham, oophorectomy (OVX)+VD deficiency (VDD), OVX, and OVX+VD supplementation (VDS). In vivo, after a 6-month intervention, imaging and pathology slice examinations showed that IDD induced by OVX was significantly alleviated in VDS and deteriorated by VDD. The expressions of aggrecan and Collagen II in intervertebral disc were reduced by OVX and VDD, and elevated by VDS. Compared with the OVX+VDD and OVX group vertebrae, OVX+VDS group vertebrae showed significantly improved endplate porosity and lumbar bone mineral density with increased percent bone volume and trabecular thickness. Furthermore, 1 alpha,25(OH)2D3 restored the redox balance (total antioxidant capacity, ratio of oxidized glutathione/glutathione) in the disc. The cocultivation of 1 alpha,25(OH)2D3 and nucleus pulposus cells (NPCs) was conducted to observe its potential ability to resist excessive oxidative stress damage induced by H2O2. In vitro experiments revealed that 1 alpha,25(OH)2D3 reduced the senescence, apoptosis, and extracellular matrix degradation induced by H2O2 in NPCs. In conclusion, VDS exhibits protective effects in OVX-induced IDD, partly by regulating the redox balance and preserving the microstructure of endplate. This finding provides a new idea for the prevention and treatment of IDD.
引用
收藏
页码:1314 / 1325
页数:12
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