Identification and Characterization of DNA-Oxaliplatin Adducts through α-hemolysin Nanopores

The antitumor effect of Pt-based drugs is determinedby their bindingactivity with deoxyribonucleic acid (DNA), and understanding the reactionprocess in a systematic manner is crucial. However, existing assaysused for DNA-Pt research suffer from several issues, such ascomplicated sample preparation, preamplification, and expensive instruments,which dramatically limit their practical application. In this study,a novel method was presented to investigate the adducts of DNA andoxaliplatin using an & alpha;-hemolysin nanopore sensor. This approachallows for real-time monitoring of the DNA-oxaliplatin condensationprocess through the detection of nanopore events associated with DNA-oxaliplatinadducts. Specifically, type I and II signals exhibiting specific currentcharacteristics were observed during the process. Typical signalswith high frequency were obtained by recording the designed DNA sequence.Furthermore, the production of these signals was confirmed to be independentof homologous adducts. This finding suggests that the DNA-oxaliplatinadduct can serve as a potential sensor for detecting oxaliplatin lesionsand multiple types of molecules.