Biocontrol Efficacy of Endophyte Pseudomonas poae to Alleviate Fusarium Seedling Blight by Refining the Morpho-Physiological Attributes of Wheat

被引：5

作者：

Ibrahim, Ezzeldin ^{[1
,2
]}

Nasser, Raghda ^{[3
,4
]}

Hafeez, Rahila ^{[1
]}

Ogunyemi, Solabomi Olaitan ^{[1
]}

Abdallah, Yasmine ^{[1
]}

Khattak, Arif Ali ^{[1
]}

Shou, Linfei ^{[5
]}

Zhang, Yang ^{[1
]}

Ahmed, Temoor ^{[1
]}

Atef Hatamleh, Ashraf ^{[6
]}

Abdullah Al-Dosary, Munirah ^{[6
]}

M. Ali, Hayssam ^{[6
]}

Luo, Jinyan ^{[7
]}

Li, Bin ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol & Breeding, Minist Agr,Key Lab Mol Biol Crop Pathogens & Insec, Hangzhou 310058, Peoples R China

[2] Agr Res Ctr, Plant Pathol Res Inst, Dept Vegetable Dis Res, Giza 12916, Egypt

[3] Zhejiang Univ, Inst Insect Sci, Coll Agr & Biotechnol, Minist Agr,Key Lab Mol Biol Crop Pathogens & Insec, Hangzhou 310058, Peoples R China

[4] Minia Univ, Fac Sci, Zool & Entomol Dept, Elminya 61519, Egypt

[5] Stn Plant Protect & Quarantine & Control Agrochem, Hangzhou 310004, Peoples R China

[6] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia

[7] Shanghai Extens & Serv Ctr Agr Technol, Dept Plant Quarantine, Shanghai 201103, Peoples R China

来源：

PLANTS-BASEL | 2023年 / 12卷 / 12期

关键词：

cell-free supernatants; deoxynivalenol; endophytes bacteria; enzymatic activities; F; graminearum; PLANT-GROWTH; HEAD BLIGHT; BACTERIA; GRAMINEARUM; IDENTIFICATION; CHITOSAN; STRAIN; AGENT; AUXIN;

D O I：

10.3390/plants12122277

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Some endophyte bacteria can improve plant growth and suppress plant diseases. However, little is known about the potential of endophytes bacteria to promote wheat growth and suppress the Fusarium seedling blight pathogen Fusarium graminearum. This study was conducted to isolate and identify endophytic bacteria and evaluate their efficacy for the plant growth promotion and disease suppression of Fusarium seedling blight (FSB) in wheat. The Pseudomonas poae strain CO showed strong antifungal activity in vitro and under greenhouse conditions against F. graminearum strain PH-1. The cell-free supernatants (CFSs) of P. poae strain CO were able to inhibit the mycelium growth, the number of colonies forming, spore germination, germ tube length, and the mycotoxin production of FSB with an inhibition rate of 87.00, 62.25, 51.33, 69.29, and 71.08%, respectively, with the highest concentration of CFSs. The results indicated that P. poae exhibited multifarious antifungal properties, such as the production of hydrolytic enzymes, siderophores, and lipopeptides. In addition, compared to untreated seeds, wheat plants treated with the strain showed significant growth rates, where root and shoot length increased by about 33% and the weight of fresh roots, fresh shoots, dry roots, and dry shoots by 50%. In addition, the strain produced high levels of indole-3-acetic acid, phosphate solubilization, and nitrogen fixation. Finally, the strain demonstrated strong antagonistic properties as well as a variety of plant growth-promoting properties. Thus, this result suggest that this strain could be used as an alternate to synthetic chemicals, which can serve as an effective method of protecting wheat from fungal infection.

引用

页数：19