Let H be a complex separable Hilbert space with $\dim H \geq 2$. Let $\mathcal {N}$ be a nest on H such that $E_+ \neq E$ for any $E \neq H, E \in \mathcal {N}$. We prove that every 2-local isometry of $\operatorname {Alg}\mathcal {N}$ is a surjective linear isometry.