Nanohybrid nanozyme based colourimetric immunosensor for porcine gelatin

被引：14

作者：

Arshad, Fareeha ^{[1
]}

Zakaria, Siti Nurul Azian ^{[1
]}

Ahmed, Minhaz Uddin ^{[1
]}

机构：

[1] Univ Brunei Darussalam, Fac Sci, Biosensors & Nanobiotechnol Lab, Integrated Sci Bldg,Jalan Tungku Link, BE-1410 Gadong, Brunei

来源：

FOOD CHEMISTRY | 2024年 / 438卷

关键词：

Nanozyme; Biosensors; Immunoassay; Immunosensor; Porcine gelatin; GOLD NANOPARTICLES; OXIDE; IMMOBILIZATION; ANTIBODIES; ELISA;

D O I：

10.1016/j.foodchem.2023.137947

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Enzyme mimicking nanomaterials, nanozymes, have gained considerable interest in the scientific community because of their superior properties compared to natural enzymes, including their high stability at extreme conditions, cheaper availability, and ease of synthesis. Herein, we report novel colloidal gold nanoparticles - graphene nanoplatelets - chitosan (CS) with peroxidase mimicking properties used to carry out highly sensitive and selective immunoassay for porcine gelatin detection. The interaction between anti-gelatin antibody conjugated nanozyme with porcine gelatin proteins produced an ultrasensitive immunoassay response in the form of a colourimetric signal directly proportional to the porcine gelatin protein concentration. The nanozyme produced a colourimetric response in the presence of its substrate, 3,3 ',5,5 '-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2), demonstrating its peroxidase mimicking properties. The results revealed that the nanozyme exhibited remarkable selectivity and sensitivity in the assay, detecting proteins at concentrations as low as 86.42 pg/mL. Additionally, the immunosensor demonstrated a broad linear detection range spanning from 200 pg/mL to 2 ng/mL.

引用

页数：11

共 73 条

[61] A sandwich ELISA for the detection of mollusks and mollusk products [J].

Tsai, Chia-Lin ;

Chen, I-Ning ;

Chen, Yi-Tien .

FOOD CHEMISTRY, 2023, 427

[62] Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody [J].

Tukiran, Nur Azira ;

Ismail, Amin ;

Mustafa, Shuhaimi ;

Hamid, Muhajir .

FOOD CONTROL, 2016, 59 :561-566

[63] Effects of gold nanoparticle morphologies on interactions with proteins [J].

Wang, Gongke ;

Wang, Wanli ;

Shangguan, Enbo ;

Gao, Shuyan ;

Liu, Yufang .

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2020, 111

[64] A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection [J].

Wang, Xiaofeng ;

Chen, Yongfeng ;

Yu, Runze ;

Wang, Ruiqiang ;

Xu, Zhixiang .

FOOD AND AGRICULTURAL IMMUNOLOGY, 2021, 32 (01) :592-605

[65] Development of a competitive ELISA based on estrogen receptor and weak competitive molecule for the screening of potential estrogens in foods [J].

Wang, Ying ;

Wang, Minglu ;

Zhou, Li ;

Geng, Xiangye ;

Xu, Zhixiang ;

Zhang, Hongyan .

FOOD CHEMISTRY, 2023, 401

[66] An Ultrasmall Fe3O4-Decorated Polydopamine Hybrid Nanozyme Enables Continuous Conversion of Oxygen into Toxic Hydroxyl Radical via GSH-Depleted Cascade Redox Reactions for Intensive Wound Disinfection [J].

Xiao, Jiayu ;

Hai, Luo ;

Li, Yaoyao ;

Li, Huan ;

Gong, Minhui ;

Wang, Zefeng ;

Tang, Zifeng ;

Deng, Le ;

He, Dinggeng .

SMALL, 2022, 18 (09)

[67] Novel nanozyme Ag/Fe3O4@h-BN with peroxidase-mimicking and oxidase-mimicking activities for dye degradation, As(V) removal and detection [J].

Xin, Ziming ;

Duan, Kaifeng ;

Zhuo, Qizheng ;

He, Qianqian ;

Zhang, Xiao ;

Zheng, Chuanjun ;

Han, Xiaoyu ;

Han, Tianfang ;

Fu, Zhongtian ;

Xu, Xinxin ;

Zhao, Xin .

CHEMICAL ENGINEERING JOURNAL, 2023, 461

[68] Bi-specific antibodies with high antigen-binding affinity identified by flow cytometry [J].

Xu, Liming ;

Zhang, Yu ;

Wang, Qiuying ;

Zhao, Jingzhuang ;

Liu, Miao ;

Guo, Mo ;

Jiang, Yuanyuan ;

Cao, Hongwei ;

Li, Qingcui ;

Ren, Guiping ;

Li, Deshan .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2015, 24 (02) :463-473

[69] Assessment of enzyme active site positioning and tests of catalytic mechanisms through X-ray-derived conformational ensembles [J].

Yabukarski, Filip ;

Biel, Justin T. ;

Pinney, Margaux M. ;

Doukov, Tzanko ;

Powers, Alexander S. ;

Fraser, James S. ;

Herschlag, Daniel .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (52) :33204-33215

[70] Detection of gelatin adulteration using bio-informatics, proteomics and high-resolution mass spectrometry [J].

Yang, Charles T. ;

Ghosh, Dipankar ;

Beaudry, Francis .

Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 2018, 35 (04) :599-608

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