Circulation of Dengue Virus Serotype 1 Genotype V and Dengue Virus Serotype 2 Genotype III in Tocantins State, Northern Brazil, 2021-2022

被引：2

作者：

de Souza, Ueric Jose Borges ^{[1
]}

Macedo, Ygor da Silva Miranda ^{[1
]}

dos Santos, Raissa Nunes ^{[1
]}

Cardoso, Franciano Dias Pereira ^{[2
]}

Galvao, Jucimaria Dantas ^{[2
]}

Gabev, Evgeni Evgeniev ^{[3
]}

Franco, Ana Claudia ^{[4
]}

Roehe, Paulo Michel ^{[4
]}

Spilki, Fernando Rosado ^{[5
]}

Campos, Fabricio Souza ^{[1
,4
]}

机构：

[1] Fed Univ Tocantins, Bioinformat & Biotechnol Lab, Campus Gurupi, BR-77410570 Gurupi, Brazil

[2] Cent Publ Hlth Lab State Tocantins, BR-77054970 Palmas, Brazil

[3] Med Univ Sofia, Dept Physiol & Pathophysiol, Sofia 1431, Bulgaria

[4] Univ Fed Rio Grande do Sul, Inst Basic Hlth Sci, Dept Microbiol Immunol & Parasitol, Virol Lab, BR-90050170 Porto Alegre, Brazil

[5] Feevale Univ, Mol Microbiol Lab, BR-93525075 Novo Hamburgo, Brazil

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 11期

关键词：

DENV; genomic surveillance; molecular clock; arboviruses; POPULATION-DYNAMICS; MOLECULAR EVOLUTION; MULTIPLEX PCR; EPIDEMIOLOGY; INFERENCE; MINION; TYPE-2; FEVER; MODEL;

D O I：

10.3390/v15112136

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In Brazil, the state of Tocantins, located in north-central Brazil, has experienced a significant number of cases of arboviral disease, particularly Dengue virus (DENV). This study aimed to deepen the knowledge on DENV circulation within that state by conducting full genome sequencing of viral genomes recovered from 61 patients between June 2021 and July 2022. There were a total of 8807 and 20,692 cases in 2021 and 2022, respectively, as reported by the state's Secretary of Health. Nucleotide sequencing confirmed the circulation of DENV serotype 1, genotype V and DENV serotype 2, genotype III in the State. Younger age groups (4 to 43 years old) were mostly affected; however, no significant differences were detected regarding the gender distribution of cases in humans. Phylogenetic analysis revealed that the circulating viruses belong to DENV-1 genotype V American and DENV-2 genotype III Southeast Asian/American. The Bayesian analysis of DENV-1 genotype V genomes sequenced here are closely related to genomes previously sequenced in the state of Sao Paulo. Regarding the DENV-2 genotype III genomes, these clustered in a distinct, well-supported subclade, along with previously reported isolates from the states of Goias and Sao Paulo. The findings reported here suggest that multiple introductions of these genotypes occurred in the Tocantins state. This observation highlights the importance of major population centers in Brazil on virus dispersion, such as those observed in other Latin American and North American countries. In the SNP analysis, DENV-1 displayed 122 distinct missense mutations, while DENV-2 had 44, with significant mutations predominantly occurring in the envelope and NS5 proteins. The analyses performed here highlight the concomitant circulation of distinct DENV-1 and -2 genotypes in some Brazilian states, underscoring the dynamic evolution of DENV and the relevance of surveillance efforts in supporting public health policies.

引用

页数：18