Physiological and transcriptomic insights into sugar stress resistance in osmophilic yeast Zygosaccharomyces rouxii

The osmophilic yeast Zygosaccharomyces rouxii has attracted increasing attention for its ability to survive and grow in extremely high sugar environments. This trait determines its role in fermentation process and results in contamination in the food industry. However, the behavior of Z. rouxii in regulating cell metabolism to combat high sugar stress and the corresponding mechanism have not been completely elucidated. Here, the resistance strategies of Z. rouxii against high glucose stress were explored by physiological analysis at cell membrane level and transcriptomic analysis. Physiological analysis showed that under high glucose stress, colony transparency increased, cell volume decreased, which was accompanied by reduction in permeability and integrity of cell membrane and subsequent gradual recovering. Additionally, the proportion of ergosterol and unsaturated fatty acids in cell membrane significantly increased under high glucose stress. A comparison of transcriptome data showed that most of the obtained differentially expressed genes (DEGs) involved in ergosterol and linoleic acid synthesis pathways as well as cell wall integrity (CWI) and high osmolarity glycerol mitogen-activated protein kinase (HOG-MAPK) pathways, which was in line with the results of physiological data. Our results provided a theoretic basis to develop the process control for the production of high sugar foods.