Rapid detection of mutations and chromosomal rearrangements in T-DNA insertion mutants of Colletotrichum higginsianum using long-read whole-genome sequencing

被引：1

作者：

Tanaka, Satomi ^{[1
]}

Yoshida, Masumi ^{[1
]}

Natsume, Hideya ^{[1
]}

Ohashi, Yuka ^{[1
]}

Takagi, Hiroki ^{[1
]}

Nishiyama, Chisato ^{[1
]}

Segawa, Tenta ^{[1
]}

Kawasaki, Yuto ^{[1
]}

Takahara, Hiroyuki ^{[1
]}

机构：

[1] Ishikawa Prefectural Univ, Fac Bioresources & Environm Sci, Dept Bioprod Sci, 1-308 Suematsu, Nonoichi, Ishikawa 9218836, Japan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2023年 / 89卷 / 04期

关键词：

Long-read whole genome sequencing; Colletotrichum higginsianum; Detection of mutation sites; T-DNA mutants; GENE; VIRULENCE;

D O I：

10.1007/s10327-023-01125-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Random mutagenesis is a useful tool for identifying genes of interest. Here, we used long-read whole-genome sequencing analysis to identify T-DNA insertion sites in pathogenicity mutants of Colletotrichum higginsianum, a fungus that causes anthracnose on Brassica plants. We identified mutated regions by aligning the sequence reads containing T-DNA to the C. higginsianum reference genome and also detected unexpectedly large chromosomal rearrangements that appear to have been caused by T-DNA transformation. These results demonstrate that long-read whole-genome sequencing is effective for identifying mutated regions in the C. higginsianum mutants.

引用

页码：206 / 210

页数：5