Unravelling the Gastroprotective Potential of Kefir: Exploring Antioxidant Effects in Preventing Gastric Ulcers

被引：2

作者：

Coco, Larissa Zambom ^{[1
]}

Aires, Rafaela ^{[1
]}

Carvalho, Glaucimeire Rocha ^{[1
]}

Belisario, Eduarda de Souza ^{[1
]}

Yap, Michelle Khai Khun ^{[2
]}

Amorim, Fernanda Gobbi ^{[3
]}

Conde-Aranda, Javier ^{[4
]}

Nogueira, Breno Valentim ^{[5
]}

Vasquez, Elisardo Corral ^{[1
]}

Pereira, Thiago de Melo Costa ^{[1
]}

Campagnaro, Bianca Prandi ^{[1
]}

机构：

[1] Vila Velha Univ UVV, Pharmaceut Sci Grad Program, Lab Translat Physiol & Pharmacol, BR-29102920 Vila Velha, ES, Brazil

[2] Monash Univ Malaysia, Sch Sci, Bandar Sunway 47500, Selangor, Malaysia

[3] Univ Liege, Dept Chem, Lab Mass Spectrometry, B-4000 Liege, Belgium

[4] Hlth Res Inst Santiago De Compostela IDIS, Mol & Cellular Gastroenterol, Santiago De Compostela 15706, Spain

[5] Fed Univ Espirito Santo UFES, Hlth Sci Ctr, Dept Morphol, BR-29047105 Vitoria, ES, Brazil

来源：

CELLS | 2023年 / 12卷 / 24期

关键词：

gastroprotection; bioprospection; oxidative stress; anti-inflammatory; OXIDATIVE STRESS; GALLIC ACID; RATS; INDOMETHACIN; CELLS; APOPTOSIS; MODEL; ROLES; INFLAMMATION; INVOLVEMENT;

D O I：

10.3390/cells12242799

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-alpha levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1-35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-alpha systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.

引用

页数：23

共 116 条

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