MiR-302c-5p affects the stemness and cisplatin resistance of nasopharyngeal carcinoma cells by regulating HSP90AA1

被引：4

作者：

Zhou, Xiangqi ^{[1
]}

Zheng, Le ^{[2
]}

Zeng, Chunya ^{[3
]}

Wu, Yangjie ^{[4
]}

Tang, Xiyang ^{[5
]}

Zhu, Yuan ^{[6
]}

Tang, Sanyuan ^{[1
,7
]}

机构：

[1] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Oncol, Hengyang, Peoples R China

[2] Xiangya Changde Hosp, Oncol Dept, Changde, Peoples R China

[3] Brain Hosp Hunan Prov, Oncol Dept, Changsha, Peoples R China

[4] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Oncol Dept, Hengyang, Peoples R China

[5] Cent South Univ, Xiangya Hosp, Dept Neurosurg, Changsha, Peoples R China

[6] Peoples Hosp Changshou Chongqing, Chongqing, Peoples R China

[7] Univ South China, Affiliated Nanhua Hosp, Hengyang Med Sch, Dept Oncol, 336 Dong Feng South Rd, Hengyang 421002, Peoples R China

来源：

ANTI-CANCER DRUGS | 2023年 / 34卷 / 01期

关键词：

AKT; cisplatin; HSP90AA1; MiR-302c-5p; Nasopharyngeal carcinoma; HEAT-SHOCK PROTEINS; CANCER; APOPTOSIS; EXPRESSION; BIOMARKERS; MICRORNAS;

D O I：

10.1097/CAD.0000000000001392

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Nasopharyngeal carcinoma (NPC) is one of the most frequent malignant tumors diagnosed in China. Cisplatin is one of the most commonly used anticancer drugs containing platinum in combined chemotherapy. The molecular mechanism of NPC is still largely unknown, and we aim to spare no effort to elucidate it. Normal human nasopharyngeal epithelial cells and NPC cell lines were cultured. The expression levels of miR-302c-5p and HSP90AA1 were detected with quantitative real-time PCR. Western blotting was used to analyze levels of the HSP90AA1, protein kinase B (AKT), p-AKT, CD44 and SOX2 proteins. The interaction between miR-302c-5p and HSP90AA1 was detected using a luciferase reporter assay. The bicinchoninic acid assay was used to observe cisplatin resistance in NPC cells. Our records confirmed that the expression of miR-302c-5p was substantially reduced and HSP90AA1 was increased in NPC cells. Additionally, miR-302c-5p inhibited cisplatin resistance and the traits of stem cells in NPC. A luciferase assay confirmed that miR-302c-5p is bound to HSP90AA1. Overexpression of HSP90AA1 may reverse the effects of overexpressed miR-302c-5p and inhibit cisplatin resistance and stem cell traits of NPC. This study investigated whether miR-302c-5p inhibited the AKT pathway by regulating HSP90AA1 expression and altered the resistance of NPC cells to cisplatin and the traits of tumor stem cells, which has not yet been reported.

引用

页码：135 / 143

页数：9