Bringing Structure to Cell Biology with Cryo-Electron Tomography

被引：47

作者：

Young, Lindsey N. ^{[1
]}

Villa, Elizabeth ^{[1
,2
]}

机构：

[1] Univ Calif San Diego, Dept Mol Biol, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92037 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS | 2023年 / 52卷

基金：

美国国家卫生研究院;

关键词：

cryo-electron tomography; in situ structural biology; quantitative cell biology; biophysical modeling; molecular architecture; MEAN FREE-PATH; IN-SITU; 3-DIMENSIONAL RECONSTRUCTION; ELECTRON TOMOGRAPHY; MICROSCOPY; COMPLEX; FLUORESCENCE; RESOLUTION; ORGANIZATION; PROTEASOMES;

D O I：

10.1146/annurev-biophys-111622-091327

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Recent advances in cryo-electron microscopy have marked only the beginning of the potential of this technique. To bring structure into cell biology, the modality of cryo-electron tomography has fast developed into a bona fide in situ structural biology technique where structures are determined in their native environment, the cell. Nearly every step of the cryo-focused ion beam-assisted electron tomography (cryo-FIB-ET) workflow has been improved upon in the past decade, since the first windows were carved into cells, unveiling macromolecular networks in near-native conditions. By bridging structural and cell biology, cryo-FIB-ET is advancing our understanding of structure-function relationships in their native environment and becoming a tool for discovering new biology.

引用

页码：573 / 595

页数：23

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