Bioinformatic analysis of wheat defensin gene family and function verification of candidate genes

被引:2
作者
Dong, Ye [1 ]
Wang, Youning [2 ]
Tang, Mingshuang [3 ]
Chen, Wang [1 ]
Chai, Yi [1 ]
Wang, Wenli [4 ]
机构
[1] Yangtze Univ, Coll Agr, MARA Key Lab Sustainable Crop Prod Middle Reaches, Jingzhou, Peoples R China
[2] Hubei Engn Univ, Hubei Key Lab Qual Control Characterist Fruits & V, Xiaogan, Hubei, Peoples R China
[3] Nanchong Acad Agr Sci, Nanchong, Sichuan, Peoples R China
[4] Northwest A&F Univ, Coll Plant Protect, Yangling, Shanxi, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2023年 / 14卷
关键词
defensins; gene structure; subcellular localization; gene expression; Phytophthora infestans infection; GENOME-WIDE IDENTIFICATION; EXPRESSION ANALYSIS; PLANT; DATABASE;
D O I
10.3389/fpls.2023.1279502
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant defensins are widely distributed in the leaves, fruits, roots, stems, seeds, and tubers. Research shows that defensin in plants play a significant role in physiological metabolism, growth and development. Plant defensins can kill and suppress a variety of pathogenic bacteria. In this study, we understand the phylogenetic relationships, protein characterization, chromosomal localization, promoter and gene structural features of the TaPDFs family through sequence alignment and conserved protein structural domain analysis. A total of 73 PDF gene members in wheat, 15 PDF genes in maize, and 11 PDF genes in rice were identified. A total of 35, 65, and 34 PDF gene members were identified in the genomes of Ae. tauschii, T. urartu, and T. dicoccoides, respectively. TaPDF4.9 and TaPDF2.15 were constructed into pART27 vector with YFP by homologous recombination for subcellular localization analysis. Subcellular localization results showed that TaPDF4.9 and TaPDF2.15 were basically located in the cell membrane and cytoplasm, and TaPDF4.9 was also located in the nucleus. TaPDF4.9 and TaPDF2.15 could inhibit the infection of Phytophthora infestans strain '88069'. The results suggest that TaPDFs may be able to improve disease resistance. The study of wheat defensins will be beneficial for improving wheat yield and provides a theoretical basis for research on resistance to wheat diseases.
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页数:14
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